TY - JOUR
T1 - Elimination of the associated microbial community and bioencapsulation of bacteria in the rotifer Brachionus plicatilis
AU - Martínez-Díaz, Sergio F.
AU - Álvarez-González, C. A.
AU - Legorreta, M. Moreno
AU - Vázquez-Juárez, Ricardo
AU - Barrios-González, Javier
N1 - Funding Information:
This work was supported by the National Council of Science and Technology CONACyT-México. The authors thank Dr F. J. Gatesoupe for critical review of this paper, Dr. B. Gomez-Gil for providing the reference strains used in this study, the personnel of the Marine Hatchery of CICIMAR for technical assistance and Manolo Magaña-Alvarez for editing this English-language text.
PY - 2003
Y1 - 2003
N2 - The bioencapsulation of live bacteria in the rotifer Brachionus plicatilis was determined under monoxenic conditions. The first objective was to evaluate the microbiota of the rotifer during intensive production and to obtain sterile rotifer cultures starting from adult females or amictic eggs using PVP-Iodine, Hydrogen peroxide or antibiotic mixtures. In the rotifers, the proportion of vibrios increased significantly during the mass production, displacing other unidentified marine bacteria. Rotifers, in the absence of culturable bacteria were obtained starting from amictic eggs and using Trimetroprim-sulfametoxasole (Bactrim Roche®) at 10 ml 1-1. The effect of members of Vibrionaceae on the survival and growth rate of rotifers was determined under monoxenic conditions. The survival of rotifers was not affected in the presence of different isolates, while amictic egg formation occurred and the populations increased when the strains Vibrio proteolyticus C279 and Aeromonas media C226 were tested. All isolates were successfully incorporated in the rotifers, since there was no significant difference between the numbers of bioencapsulated cells of different strains of isolates. The results show that it is possible to replace the microbial community in rotifer cultures, started from disinfected amictic eggs, with selected bacterial strains. This could be used as a tool for future studies to reveal the role of specific bacteria on first larval stages of marine fish species.
AB - The bioencapsulation of live bacteria in the rotifer Brachionus plicatilis was determined under monoxenic conditions. The first objective was to evaluate the microbiota of the rotifer during intensive production and to obtain sterile rotifer cultures starting from adult females or amictic eggs using PVP-Iodine, Hydrogen peroxide or antibiotic mixtures. In the rotifers, the proportion of vibrios increased significantly during the mass production, displacing other unidentified marine bacteria. Rotifers, in the absence of culturable bacteria were obtained starting from amictic eggs and using Trimetroprim-sulfametoxasole (Bactrim Roche®) at 10 ml 1-1. The effect of members of Vibrionaceae on the survival and growth rate of rotifers was determined under monoxenic conditions. The survival of rotifers was not affected in the presence of different isolates, while amictic egg formation occurred and the populations increased when the strains Vibrio proteolyticus C279 and Aeromonas media C226 were tested. All isolates were successfully incorporated in the rotifers, since there was no significant difference between the numbers of bioencapsulated cells of different strains of isolates. The results show that it is possible to replace the microbial community in rotifer cultures, started from disinfected amictic eggs, with selected bacterial strains. This could be used as a tool for future studies to reveal the role of specific bacteria on first larval stages of marine fish species.
KW - Bioencapsulation
KW - Gnotobiotic rotifers
KW - Rotifer microbiota
KW - Vibrionaceae
UR - http://www.scopus.com/inward/record.url?scp=18144451159&partnerID=8YFLogxK
U2 - 10.1023/A:1024117109362
DO - 10.1023/A:1024117109362
M3 - Artículo
SN - 0967-6120
VL - 11
SP - 95
EP - 108
JO - Aquaculture International
JF - Aquaculture International
IS - 1-2
ER -