TY - JOUR
T1 - Electron probe analysis and biochemical characterization of electron-dense granules secreted by Entamoeba histolytica
AU - León, Gloria
AU - Fiori, Charles
AU - Das, Pradeep
AU - Moreno, Miguel
AU - Tovar, Rosalinda
AU - Sánchez-Salas, José Luis
AU - De Lourdes Muñoz, María
N1 - Funding Information:
This work was supported, in part, by grants 211085-5-0119PN and F547N-9309 from CONA-CyT, Mexico. We thank Richard Leapman and Dr Arturo Ortega for critical reading of this manuscript, Dr Jos6 Manuel Hernfindez for the donation of the anti-actin mab and the staff of the Electron Microscopy Unit CINVESTAV, IPN, for use of their facilities.
PY - 1997/4
Y1 - 1997/4
N2 - The interaction of Entamoeba histolytica with collagen induces the intracellular formation and release of electron-dense granules (EDGs) containing collagenase activity which are important in the pathogenicity of this parasite. Purified EDGs contain at least 25 polypeptides with acidic pIs, nine gelatinase activities, small molecules, including inorganic phosphate (P(i)), pyrophosphate (PP) and other elements, including Na, Mg, S, Cl, K, Ca and Fe as measured by scanning transmission electron microscopy. Six of these polypeptides with apparent molecular weights of 108, 106, 104, 97, 68 and 59 kDa and two protease activities with apparent molecular weights of 40 and 85 kDa were detected exclusively in the EDGs and were not observed in total trophozoite extracts. Actin was also detected in the EDGs. Therefore, EDGs are a complex of mainly cationic proteins, which contains numerous proteolytic activities, actin and small molecules such as P(i), PP and cations.
AB - The interaction of Entamoeba histolytica with collagen induces the intracellular formation and release of electron-dense granules (EDGs) containing collagenase activity which are important in the pathogenicity of this parasite. Purified EDGs contain at least 25 polypeptides with acidic pIs, nine gelatinase activities, small molecules, including inorganic phosphate (P(i)), pyrophosphate (PP) and other elements, including Na, Mg, S, Cl, K, Ca and Fe as measured by scanning transmission electron microscopy. Six of these polypeptides with apparent molecular weights of 108, 106, 104, 97, 68 and 59 kDa and two protease activities with apparent molecular weights of 40 and 85 kDa were detected exclusively in the EDGs and were not observed in total trophozoite extracts. Actin was also detected in the EDGs. Therefore, EDGs are a complex of mainly cationic proteins, which contains numerous proteolytic activities, actin and small molecules such as P(i), PP and cations.
KW - Entamoeba histolytica
KW - actin
KW - collagenase
KW - electron dense-granules
KW - electron-probe analysis
KW - protease
KW - secretion
UR - http://www.scopus.com/inward/record.url?scp=0343683326&partnerID=8YFLogxK
U2 - 10.1016/S0166-6851(97)02833-8
DO - 10.1016/S0166-6851(97)02833-8
M3 - Artículo
SN - 0166-6851
VL - 85
SP - 233
EP - 242
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -