Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process

C. I. Guerrero-Robles; G. J. Vazquez-Zapien; M. M. Mata-Miranda; J. E. Noriega-Gonzalez; C. A. Gonzalez-Diaz

doi:10.1109/EMBC.2017.8037628

Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process

C. I. Guerrero-Robles, G. J. Vazquez-Zapien, M. M. Mata-Miranda, J. E. Noriega-Gonzalez, C. A. Gonzalez-Diaz

Escuela Superior de Medicina (ESM)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

The identification and characterization of diverse cells types and cell differentiation process requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyze the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as a non-complex alternative technique to identify populations of undifferentiated mouse Pluripotent Stem Cells (mPSCs), Mouse Embryonic Fibroblasts (MEFs) and the differentiation process from preadipocytes (3T3-L1) to mature adipocytes. EBIS measurements were compared in populations of cells which were characterized previously using microscopy. The results indicate that EBIS technique has a potential sensitivity at certain frequency range to discriminate between both evaluated cell populations and some differentiation process. Additional studies with different concentrations to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.

Original language	English
Title of host publication	2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Subtitle of host publication	Smarter Technology for a Healthier World, EMBC 2017 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	3568-3571
Number of pages	4
ISBN (Electronic)	9781509028092
DOIs	https://doi.org/10.1109/EMBC.2017.8037628
State	Published - 13 Sep 2017
Event	39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2017 - Jeju Island, Korea, Republic of Duration: 11 Jul 2017 → 15 Jul 2017

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)	1557-170X

Conference

Conference	39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2017
Country/Territory	Korea, Republic of
City	Jeju Island
Period	11/07/17 → 15/07/17

Access to Document

10.1109/EMBC.2017.8037628

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Guerrero-Robles, C. I., Vazquez-Zapien, G. J., Mata-Miranda, M. M., Noriega-Gonzalez, J. E., & Gonzalez-Diaz, C. A. (2017). Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. In 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings (pp. 3568-3571). Article 8037628 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2017.8037628

Guerrero-Robles, C. I. ; Vazquez-Zapien, G. J. ; Mata-Miranda, M. M. et al. / Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 3568-3571 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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abstract = "The identification and characterization of diverse cells types and cell differentiation process requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyze the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as a non-complex alternative technique to identify populations of undifferentiated mouse Pluripotent Stem Cells (mPSCs), Mouse Embryonic Fibroblasts (MEFs) and the differentiation process from preadipocytes (3T3-L1) to mature adipocytes. EBIS measurements were compared in populations of cells which were characterized previously using microscopy. The results indicate that EBIS technique has a potential sensitivity at certain frequency range to discriminate between both evaluated cell populations and some differentiation process. Additional studies with different concentrations to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.",

author = "Guerrero-Robles, {C. I.} and Vazquez-Zapien, {G. J.} and Mata-Miranda, {M. M.} and Noriega-Gonzalez, {J. E.} and Gonzalez-Diaz, {C. A.}",

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Guerrero-Robles, CI, Vazquez-Zapien, GJ, Mata-Miranda, MM, Noriega-Gonzalez, JE & Gonzalez-Diaz, CA 2017, Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. in 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings., 8037628, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, Institute of Electrical and Electronics Engineers Inc., pp. 3568-3571, 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2017, Jeju Island, Korea, Republic of, 11/07/17. https://doi.org/10.1109/EMBC.2017.8037628

Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. / Guerrero-Robles, C. I.; Vazquez-Zapien, G. J.; Mata-Miranda, M. M. et al.
2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. p. 3568-3571 8037628 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Guerrero-Robles, C. I.

AU - Vazquez-Zapien, G. J.

AU - Mata-Miranda, M. M.

AU - Noriega-Gonzalez, J. E.

AU - Gonzalez-Diaz, C. A.

PY - 2017/9/13

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N2 - The identification and characterization of diverse cells types and cell differentiation process requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyze the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as a non-complex alternative technique to identify populations of undifferentiated mouse Pluripotent Stem Cells (mPSCs), Mouse Embryonic Fibroblasts (MEFs) and the differentiation process from preadipocytes (3T3-L1) to mature adipocytes. EBIS measurements were compared in populations of cells which were characterized previously using microscopy. The results indicate that EBIS technique has a potential sensitivity at certain frequency range to discriminate between both evaluated cell populations and some differentiation process. Additional studies with different concentrations to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.

AB - The identification and characterization of diverse cells types and cell differentiation process requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyze the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as a non-complex alternative technique to identify populations of undifferentiated mouse Pluripotent Stem Cells (mPSCs), Mouse Embryonic Fibroblasts (MEFs) and the differentiation process from preadipocytes (3T3-L1) to mature adipocytes. EBIS measurements were compared in populations of cells which were characterized previously using microscopy. The results indicate that EBIS technique has a potential sensitivity at certain frequency range to discriminate between both evaluated cell populations and some differentiation process. Additional studies with different concentrations to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.

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ER -

Guerrero-Robles CI, Vazquez-Zapien GJ, Mata-Miranda MM, Noriega-Gonzalez JE, Gonzalez-Diaz CA. Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. In 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2017. p. 3568-3571. 8037628. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2017.8037628

Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process

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