TY - JOUR
T1 - Efficient recovery of thermostable polyhydroxybutyrate (PHB) by a rapid and solvent-free extraction protocol assisted by ultrasound
AU - Martínez-Herrera, Raul E.
AU - Alemán-Huerta, María E.
AU - Almaguer-Cantú, Verónica
AU - Rosas-Flores, Walfred
AU - Martínez-Gómez, Víctor J.
AU - Quintero-Zapata, Isela
AU - Rivera, Gildardo
AU - Rutiaga-Quiñones, O. Miriam
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Nowadays, there are great research interest in polyhydroxybutyrate (PHB) recovery protocols that reduce the use of organic solvents and efficiently recover this bacterial biopolymer. The present study reports an extraction protocol assisted by ultrasound, which is a rapid protocol that increases the amount of polymeric matter extracted, reduces the cellular digestion step with sodium hypochlorite and eliminates the use of organic solvents. Likewise, characterization studies by Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance spectroscopy (1H NMR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) demonstrated that the biopolymer recovered by this protocol is PHB homopolymer with a major thermal-resistance and crystalline properties. Besides, in this study are reported the thermic and crystalline differences between the PHB obtained from the fermentation of complex carbohydrates (agavins) and simple sugars. The biopolymer obtained by this rapid extraction protocol would be suitable for ecological and biomedical applications, due to the low melting temperature, less than 50% crystallinity, and the lack of lipopolysaccharides. Therefore, this extraction protocol might represent an alternative to the traditional protocol based on NaOCl-chloroform and is part of the green trend to improve the PHB production.
AB - Nowadays, there are great research interest in polyhydroxybutyrate (PHB) recovery protocols that reduce the use of organic solvents and efficiently recover this bacterial biopolymer. The present study reports an extraction protocol assisted by ultrasound, which is a rapid protocol that increases the amount of polymeric matter extracted, reduces the cellular digestion step with sodium hypochlorite and eliminates the use of organic solvents. Likewise, characterization studies by Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance spectroscopy (1H NMR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) demonstrated that the biopolymer recovered by this protocol is PHB homopolymer with a major thermal-resistance and crystalline properties. Besides, in this study are reported the thermic and crystalline differences between the PHB obtained from the fermentation of complex carbohydrates (agavins) and simple sugars. The biopolymer obtained by this rapid extraction protocol would be suitable for ecological and biomedical applications, due to the low melting temperature, less than 50% crystallinity, and the lack of lipopolysaccharides. Therefore, this extraction protocol might represent an alternative to the traditional protocol based on NaOCl-chloroform and is part of the green trend to improve the PHB production.
KW - Agavins
KW - Biopolymers
KW - Polyhydroxyalkanoates
KW - Ultrasound-assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85088402189&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2020.07.101
DO - 10.1016/j.ijbiomac.2020.07.101
M3 - Artículo
C2 - 32682039
AN - SCOPUS:85088402189
SN - 0141-8130
VL - 164
SP - 771
EP - 782
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -