TY - GEN
T1 - Efficiency of the photodynamic therapy using gold nanoparticles (np-Au) and PpIX induced and not induced
AU - Elizabeth, Maldonado Alvarado
AU - Eva, Ramón Gallegos
AU - Judith, Tánori Córdova
AU - Jesús, Arenas Huertero Francisco
AU - Esther, Sánchez Espíndola María
AU - Alicia, Reyes Arellano
AU - Luis, Jiménez Pérez José
AU - Alfredo, Cruz Orea
PY - 2008
Y1 - 2008
N2 - The use of gold nanoparticles (np-Au) to eliminate cancer has proved to be very effective due to the fact that cancerous cells accumulate it 600% more than healthy cells. In addition they have a high capacity of absorption and dispersion of light. Therefore, the effectiveness of photodynamic therapy (PDT) could be improved by the simultaneous use of np-Au and photosensitizes (Ps), emphasizing the high efficiency of the PDT to diagnose and to treat pre-malignant and malignant processes. The aim of this work was to determine the efficiency of PDT using np-Au and protoporphyrin IX (PpIX) induced and not induced by the δ-aminolevulinic acid (ALA). It were found the conditions of synthesis of hydrosoluble np-Au, and were characterized by transmission electronic microscopy (TEM) and UV-VIS spectroscopy. It was realized a kinetic by TEM to determine the cellular incorporation time of np-Au, the maximum incorporation of np-Au was of 1.6 h. PDT was applied using different doses of np-Au and photosensitizers. It was observed that the use of PDT simultaneously with np-Au did not increase the mortality of HeLa cells. In the case of C33, when PpIX not induced is used as photosensitizer simultaneously with np-Au, the mortality increased 20%.
AB - The use of gold nanoparticles (np-Au) to eliminate cancer has proved to be very effective due to the fact that cancerous cells accumulate it 600% more than healthy cells. In addition they have a high capacity of absorption and dispersion of light. Therefore, the effectiveness of photodynamic therapy (PDT) could be improved by the simultaneous use of np-Au and photosensitizes (Ps), emphasizing the high efficiency of the PDT to diagnose and to treat pre-malignant and malignant processes. The aim of this work was to determine the efficiency of PDT using np-Au and protoporphyrin IX (PpIX) induced and not induced by the δ-aminolevulinic acid (ALA). It were found the conditions of synthesis of hydrosoluble np-Au, and were characterized by transmission electronic microscopy (TEM) and UV-VIS spectroscopy. It was realized a kinetic by TEM to determine the cellular incorporation time of np-Au, the maximum incorporation of np-Au was of 1.6 h. PDT was applied using different doses of np-Au and photosensitizers. It was observed that the use of PDT simultaneously with np-Au did not increase the mortality of HeLa cells. In the case of C33, when PpIX not induced is used as photosensitizer simultaneously with np-Au, the mortality increased 20%.
KW - Cancer
KW - Nanoparticles
KW - PDT
KW - Photosensitizer
UR - http://www.scopus.com/inward/record.url?scp=52649096884&partnerID=8YFLogxK
U2 - 10.1063/1.2979296
DO - 10.1063/1.2979296
M3 - Contribución a la conferencia
AN - SCOPUS:52649096884
SN - 9780735405561
T3 - AIP Conference Proceedings
SP - 295
EP - 296
BT - Medical Physics - Tenth Mexican Symposium on Medical Physics
T2 - 10th Mexican Symposium on Medical Physics
Y2 - 17 March 2008 through 19 March 2008
ER -
