TY - JOUR
T1 - Effects of the toxic dinoflagellate, Gymnodinium catenatum on hydrolytic and antioxidant enzymes, in tissues of the giant lions-paw scallop Nodipecten subnodosus
AU - Estrada, Norma
AU - de Jesús Romero, Maria
AU - Campa-Córdova, Angel
AU - Luna, Antonio
AU - Ascencio, Felipe
N1 - Funding Information:
We thank C. Rodríguez for her assistance with histopathological records. Financial support was provided by Centro de Investigaciones Biológicas del Noroeste (CIBNOR grant AC 3.1) and Consejo Nacional de Ciencia y Tecnología of Mexico (CONACYT fellowship 172583) to the first author.
PY - 2007/11
Y1 - 2007/11
N2 - This study documents effects of the toxic dinoflagellate Gymnodinium catenatum, a producer of paralytic shellfish poison, on juvenile farmed (5.9 ± 0.39 cm) giant lions-paw scallop Nodipecten subnodosus. Scallops were fed bloom concentrations of toxic dinoflagellate G. catenatum for 7 h. The effect of the toxic dinoflagellate in different tissues was determined by analysis of antioxidant enzymes (catalase, superoxide dismutase, gluthathione peroxidase), thiobarbituric acid reactive substances (lipid peroxidation), and hydrolytic enzymes (proteases, glycosidases, phosphatases, lipases, and esterases). Histopathological photos record the effects of the toxic dinoflagellate in various tissues. The results show that juvenile lions-paw scallops produce pseudo-feces, partially close their shell, increase melanization, and aggregate hemocytes. Several enzymes were affected and could serve as biological markers. In general, the adductor muscle was not affected. In the digestive gland, some enzymes could be the result of defensive and digestive processes. Gills and mantle tissue were markedly affected because these sites respond first to toxic dinoflagellates, leading to the idea that proteolytic cascades could be involved.
AB - This study documents effects of the toxic dinoflagellate Gymnodinium catenatum, a producer of paralytic shellfish poison, on juvenile farmed (5.9 ± 0.39 cm) giant lions-paw scallop Nodipecten subnodosus. Scallops were fed bloom concentrations of toxic dinoflagellate G. catenatum for 7 h. The effect of the toxic dinoflagellate in different tissues was determined by analysis of antioxidant enzymes (catalase, superoxide dismutase, gluthathione peroxidase), thiobarbituric acid reactive substances (lipid peroxidation), and hydrolytic enzymes (proteases, glycosidases, phosphatases, lipases, and esterases). Histopathological photos record the effects of the toxic dinoflagellate in various tissues. The results show that juvenile lions-paw scallops produce pseudo-feces, partially close their shell, increase melanization, and aggregate hemocytes. Several enzymes were affected and could serve as biological markers. In general, the adductor muscle was not affected. In the digestive gland, some enzymes could be the result of defensive and digestive processes. Gills and mantle tissue were markedly affected because these sites respond first to toxic dinoflagellates, leading to the idea that proteolytic cascades could be involved.
KW - Antioxidant enzymes
KW - Gymnodinium catenatum
KW - Histopathology
KW - Hydrolytic enzymes
KW - Lipid peroxidation
KW - Nodipecten subnodosus
UR - http://www.scopus.com/inward/record.url?scp=34848825900&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2007.06.003
DO - 10.1016/j.cbpc.2007.06.003
M3 - Artículo
C2 - 17613278
AN - SCOPUS:34848825900
SN - 1532-0456
VL - 146
SP - 502
EP - 510
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 4
ER -