TY - JOUR
T1 - Effects of hydroxyurea on monoclonal antibody production induced by anti-mIgG and LPS stimulation on murine B cell hybridomas
AU - Martín-López, Alicia
AU - Sánchez-Mirón, Asterio
AU - García-Camacho, Francisco
AU - Contreras-Gómez, Antonio
AU - Molina-Grima, Emilio
N1 - Funding Information:
Acknowledgments We would like to thank the Spanish Ministry for Education and Science for conceding a Postgraduate Grant to A. Martín-López that has made this research possible. The authors acknowledge and appreciate the financial support received from Junta de Andalucía-Spain (P07-CVI-03193) and Ministerio de Ciencia e Innovación-Spain (BIO2008-06505).
PY - 2010/6
Y1 - 2010/6
N2 - Chemical treatment with hydroxyurea (HU) has been selected as a simple and low cost strategy to generate a cell population enriched for the G1 phase. After the chemical treatment with HU, cells were stimulated with anti-mIgG to test if the positive effects of anti-mIgG on CD40 expression and specific IgG2a production rate were improved upon a cell population with a higher percentage of cells in G1 phase at the beginning of the cell culture. In addition, other treatments assayed in this work were the cell stimulation with Lipopolysaccharide (LPS) both before and after the HU treatment. It has been observed that the use of HU under conditions able to maintain the cells in viable state (0.1 mM for 20 h), has a negative effect on CD40 expression and specific IgG2a production rate induced by anti-mIgG. The positive effect of LPS on cell stimulation induced by anti-mIgG is reduced on cells treated with HU.
AB - Chemical treatment with hydroxyurea (HU) has been selected as a simple and low cost strategy to generate a cell population enriched for the G1 phase. After the chemical treatment with HU, cells were stimulated with anti-mIgG to test if the positive effects of anti-mIgG on CD40 expression and specific IgG2a production rate were improved upon a cell population with a higher percentage of cells in G1 phase at the beginning of the cell culture. In addition, other treatments assayed in this work were the cell stimulation with Lipopolysaccharide (LPS) both before and after the HU treatment. It has been observed that the use of HU under conditions able to maintain the cells in viable state (0.1 mM for 20 h), has a negative effect on CD40 expression and specific IgG2a production rate induced by anti-mIgG. The positive effect of LPS on cell stimulation induced by anti-mIgG is reduced on cells treated with HU.
KW - Anti-mIgG
KW - CD40
KW - Hydroxyurea
KW - Lipopolysaccharide
KW - Murine B cell hybridoma
UR - http://www.scopus.com/inward/record.url?scp=77956897383&partnerID=8YFLogxK
U2 - 10.1007/s10616-010-9278-9
DO - 10.1007/s10616-010-9278-9
M3 - Artículo
AN - SCOPUS:77956897383
SN - 0920-9069
VL - 62
SP - 205
EP - 215
JO - Cytotechnology
JF - Cytotechnology
IS - 3
ER -