TY - JOUR
T1 - Effects of DNA damage induced by UV irradiation on gene expression in the protozoan parasite Entamoeba histolytica
AU - Weber, Christian
AU - Marchat, Laurence A.
AU - Guillen, Nancy
AU - López-Camarillo, César
N1 - Funding Information:
We thank Jean Yves Copee, Odile Sismeiro and Ghislaine Guigon for their help with microarray analysis. We also thank Sollange Archer for artwork assistance. This work was supported by a grant from the Pasteur-Weizmann Research Council and Mexican grants from CONACyT, UACM, COFFA-IPN, and SIP-IPN. Cesar Lopez-Camarillo was recipient of a fellowship from the French Embassy in Mexico.
PY - 2009/4
Y1 - 2009/4
N2 - Previously, we provided evidence for the role of E. histolytica RAD52 epistasis group genes and the EhRAD51 recombinase in DNA damage response. To identify other genes participating in DNA repair in this protozoan parasite, here we analyzed the transcriptional response to genetic damage induced by ultraviolet light (UV) using cDNA microarrays. We found that 11.6% (350 ORFs) and 17.2% (522 ORFs) of genes were modulated at 5 min and 3 h after UV irradiation, respectively. Most genes were less than 2-fold changed evidencing a weak transcriptional activation. The genes encoding so-called "classical" DNA repair proteins were slightly regulated in trophozoites submitted to UV irradiation. We also observed the over-expression of genes encoding for Fe-S clusters-containing proteins, potentially involved in the stress adaptation in response to DNA damage. Several genes encoding cytoskeleton proteins were repressed suggesting that actin dynamics was impaired after UV irradiation. Our analysis highlights novel genes potentially involved in DNA damage response, and these data will contribute to further elucidation of mechanisms regulating genome integrity in this early branch protozoan.
AB - Previously, we provided evidence for the role of E. histolytica RAD52 epistasis group genes and the EhRAD51 recombinase in DNA damage response. To identify other genes participating in DNA repair in this protozoan parasite, here we analyzed the transcriptional response to genetic damage induced by ultraviolet light (UV) using cDNA microarrays. We found that 11.6% (350 ORFs) and 17.2% (522 ORFs) of genes were modulated at 5 min and 3 h after UV irradiation, respectively. Most genes were less than 2-fold changed evidencing a weak transcriptional activation. The genes encoding so-called "classical" DNA repair proteins were slightly regulated in trophozoites submitted to UV irradiation. We also observed the over-expression of genes encoding for Fe-S clusters-containing proteins, potentially involved in the stress adaptation in response to DNA damage. Several genes encoding cytoskeleton proteins were repressed suggesting that actin dynamics was impaired after UV irradiation. Our analysis highlights novel genes potentially involved in DNA damage response, and these data will contribute to further elucidation of mechanisms regulating genome integrity in this early branch protozoan.
KW - Amoebiasis
KW - DNA damage repair
KW - Double strand breaks
KW - Entamoeba histolytica
KW - cDNA microarrays
UR - http://www.scopus.com/inward/record.url?scp=59649083186&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2008.12.005
DO - 10.1016/j.molbiopara.2008.12.005
M3 - Artículo
SN - 0166-6851
VL - 164
SP - 165
EP - 169
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -