TY - JOUR
T1 - Effect of UV and gamma irradiation sterilization processes in the properties of different polymeric nanoparticles for biomedical applications
AU - Tapia-Guerrero, Y. S.
AU - Del Prado-Audelo, M. L.
AU - Borbolla-Jiménez, F. V.
AU - Giraldo Gomez, D. M.
AU - García-Aguirre, I.
AU - Colín-Castro, C. A.
AU - Morales-González, J. A.
AU - Leyva-Gómez, G.
AU - Magaña, J. J.
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(ε-caprolactone) and poly(D, L-lactide-co-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre-and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 x 108 CFU/mL of Escherichia coli, Staphylococcus aureus, and Candida albicans. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 μJ/cm2), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 μg/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reducesthe cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems.
AB - The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(ε-caprolactone) and poly(D, L-lactide-co-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre-and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 x 108 CFU/mL of Escherichia coli, Staphylococcus aureus, and Candida albicans. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 μJ/cm2), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 μg/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reducesthe cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems.
KW - Gamma radiation
KW - Nanoparticles
KW - Poly (d, l-lactide-coglycolide) acid
KW - Poly-('-caprolactone)
KW - Sterilization
KW - Ultraviolet radiation
UR - http://www.scopus.com/inward/record.url?scp=85081693624&partnerID=8YFLogxK
U2 - 10.3390/ma13051090
DO - 10.3390/ma13051090
M3 - Artículo
AN - SCOPUS:85081693624
SN - 1996-1944
VL - 13
JO - Materials
JF - Materials
IS - 5
M1 - 1090
ER -