TY - JOUR
T1 - Effect of TiO2-ZnO-MgO mixed oxide on microbial growth and toxicity against artemia salina
AU - Anaya-Esparza, Luis M.
AU - González-Silva, Napoleón
AU - Yahia, Elhadi M.
AU - González-Vargas, O. A.
AU - Montalvo-González, Efigenia
AU - Pérez-Larios, Alejandro
N1 - Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/7
Y1 - 2019/7
N2 - Mixed oxide nanoparticles (MONs, TiO2–ZnO–MgO) obtained by the sol-gel method were characterized by transmission electron microscopy, (TEM,HRTEM, and SAED) and thermogravimetric analysis (TGA/DTGA–DTA). Furthermore, the effect of MONs on microbial growth (growth profiling curve, lethal and sublethal effect) of Escherichia coli, Salmonella paratyphi, Staphylococcus aureus and Listeria monocytogenes, as well as the toxicity against Artemia salina by the lethal concentration test (LC50) were evaluated. MONs exhibited a near-spherical in shape, polycrystalline structure and mean sizes from 17 to 23 nm. The thermal analysis revealed that the anatase phase ofMONs is completed around 480–500 °C. The normal growth of all bacteria tested is affected by theMONs presence compared with the control group. MONs also exhibited a reduction on the plate count from 0.58 to 2.10 log CFU/mL with a sublethal cell injury from 17 to 98%. No significant toxicity within 24 h was observed on A. salina. A bacteriostatic effect ofMONs on bacteria was evidenced, which was strongly influenced by the type of bacteria, as well as no toxic effects (LC50 >1000 mg/L; TiO2–ZnO (5%)–MgO (5%)) on A. salina were detected. This study demonstrates the potential ofMONs for industrial applications.
AB - Mixed oxide nanoparticles (MONs, TiO2–ZnO–MgO) obtained by the sol-gel method were characterized by transmission electron microscopy, (TEM,HRTEM, and SAED) and thermogravimetric analysis (TGA/DTGA–DTA). Furthermore, the effect of MONs on microbial growth (growth profiling curve, lethal and sublethal effect) of Escherichia coli, Salmonella paratyphi, Staphylococcus aureus and Listeria monocytogenes, as well as the toxicity against Artemia salina by the lethal concentration test (LC50) were evaluated. MONs exhibited a near-spherical in shape, polycrystalline structure and mean sizes from 17 to 23 nm. The thermal analysis revealed that the anatase phase ofMONs is completed around 480–500 °C. The normal growth of all bacteria tested is affected by theMONs presence compared with the control group. MONs also exhibited a reduction on the plate count from 0.58 to 2.10 log CFU/mL with a sublethal cell injury from 17 to 98%. No significant toxicity within 24 h was observed on A. salina. A bacteriostatic effect ofMONs on bacteria was evidenced, which was strongly influenced by the type of bacteria, as well as no toxic effects (LC50 >1000 mg/L; TiO2–ZnO (5%)–MgO (5%)) on A. salina were detected. This study demonstrates the potential ofMONs for industrial applications.
KW - Antimicrobial activity
KW - Artemia salina
KW - Mixed oxides
KW - Nanomaterials
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=85073296417&partnerID=8YFLogxK
U2 - 10.3390/nano9070992
DO - 10.3390/nano9070992
M3 - Artículo
C2 - 31295802
SN - 2079-4991
VL - 9
JO - Nanomaterials
JF - Nanomaterials
IS - 7
M1 - 992
ER -