TY - JOUR
T1 - Effect of sodium chloride concentration on growth and degradation of eicosane by the Marine halotolerant bacterium Marinobacter hydrocarbonoclasticus
AU - Fernandez-Linares, Luis
AU - Acquaviva, Monique
AU - Bertrand, Jean Claude
AU - Gauthier, Michel
PY - 1996/3
Y1 - 1996/3
N2 - The extremely halotolerant marine species Marinobacter hydrocarbonoclastictis presents an absolute requirement for NA+ ions. When grown on acetate or eicosane, lag phase of cultures and generation time of cells increased exponentially at NaCl concentrations ≥ 1.0 M whereas the final biomass did not vary significantly (p ≤ 0.05) when salinity increased from 0.2 to 2.5 M NaCl. The increase in salinity induced morphological changes with increased cell size. Scanning electron microscopy revealed extracellular vesicles on cells grown on eicosane. High salinity did not modify hydrocarbon biodegradation rates (80-90% biodegraded eicosane). This bacterium produced an extracellular emulsifying agent and adhered to hydrocarbon, but did not solubilize it before uptake. Both emulsification and adherence took place during growth on eicosane, achieving contact between cells and hydrocarbon. An increase in external NaCl concentration modified emulsifying activity and adherence ability, but not the biodegradation rates.
AB - The extremely halotolerant marine species Marinobacter hydrocarbonoclastictis presents an absolute requirement for NA+ ions. When grown on acetate or eicosane, lag phase of cultures and generation time of cells increased exponentially at NaCl concentrations ≥ 1.0 M whereas the final biomass did not vary significantly (p ≤ 0.05) when salinity increased from 0.2 to 2.5 M NaCl. The increase in salinity induced morphological changes with increased cell size. Scanning electron microscopy revealed extracellular vesicles on cells grown on eicosane. High salinity did not modify hydrocarbon biodegradation rates (80-90% biodegraded eicosane). This bacterium produced an extracellular emulsifying agent and adhered to hydrocarbon, but did not solubilize it before uptake. Both emulsification and adherence took place during growth on eicosane, achieving contact between cells and hydrocarbon. An increase in external NaCl concentration modified emulsifying activity and adherence ability, but not the biodegradation rates.
KW - Adherence
KW - Biodegradation
KW - Eicosane
KW - Emulsifying activity
KW - Growth
KW - Marine halotolerant bacterium
KW - Salinity
UR - http://www.scopus.com/inward/record.url?scp=0029869171&partnerID=8YFLogxK
U2 - 10.1016/S0723-2020(96)80018-X
DO - 10.1016/S0723-2020(96)80018-X
M3 - Artículo
AN - SCOPUS:0029869171
SN - 0723-2020
VL - 19
SP - 113
EP - 121
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 1
ER -