Effect of sample solubilized aliquot volume and concentration on hydroperoxide detection in PUFA-rich fish oil

Violeta Ugalde-Benítez; Arturo Rojo-Domínguez; María Eugenia Jaramillo-Flores; Isabel Guerrero-Legarreta

doi:10.1080/10942912.2012.660719

Effect of sample solubilized aliquot volume and concentration on hydroperoxide detection in PUFA-rich fish oil

Violeta Ugalde-Benítez, Arturo Rojo-Domínguez, María Eugenia Jaramillo-Flores, Isabel Guerrero-Legarreta

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The objective of this work was to study the effect of sample oil concentration and volume on hydroperoxide determination in n-3 PUFA-rich sardine-anchovy oil. Oil samples were stored at-20°C, 6 d, air-free (T1); and 20°C, 2 d in air (T2). Significant differences between storage conditions were observed on hydroperoxide concentration. High reproducibility was obtained even in sample concentrations as low as 5 mg oil/mL of propan-1-ol. A high correlation was observed between the modified ferrous oxidation-xylenol orange method (FOX2) and AOAC peroxide value analysis methods, although FOX2 was faster, more sensitive, and precise when analyzing small hydroperoxide concentrations.

Original language	English
Pages (from-to)	650-660
Number of pages	11
Journal	International Journal of Food Properties
Volume	17
Issue number	3
DOIs	https://doi.org/10.1080/10942912.2012.660719
State	Published - 16 Mar 2014

Keywords

Fish oil
Hydroperoxides
Lipid oxidation
n-3 Fatty acids

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10.1080/10942912.2012.660719

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title = "Effect of sample solubilized aliquot volume and concentration on hydroperoxide detection in PUFA-rich fish oil",

abstract = "The objective of this work was to study the effect of sample oil concentration and volume on hydroperoxide determination in n-3 PUFA-rich sardine-anchovy oil. Oil samples were stored at-20°C, 6 d, air-free (T1); and 20°C, 2 d in air (T2). Significant differences between storage conditions were observed on hydroperoxide concentration. High reproducibility was obtained even in sample concentrations as low as 5 mg oil/mL of propan-1-ol. A high correlation was observed between the modified ferrous oxidation-xylenol orange method (FOX2) and AOAC peroxide value analysis methods, although FOX2 was faster, more sensitive, and precise when analyzing small hydroperoxide concentrations.",

keywords = "Fish oil, Hydroperoxides, Lipid oxidation, n-3 Fatty acids",

author = "Violeta Ugalde-Ben{\'i}tez and Arturo Rojo-Dom{\'i}nguez and Jaramillo-Flores, {Mar{\'i}a Eugenia} and Isabel Guerrero-Legarreta",

note = "Funding Information: Violeta Ugalde-Ben{\'i}tez wishes to thank the National Council of Science and Technology (CONACYT, Mexico) for a graduate scholarship.",

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AU - Ugalde-Benítez, Violeta

AU - Rojo-Domínguez, Arturo

AU - Jaramillo-Flores, María Eugenia

AU - Guerrero-Legarreta, Isabel

N1 - Funding Information: Violeta Ugalde-Benítez wishes to thank the National Council of Science and Technology (CONACYT, Mexico) for a graduate scholarship.

PY - 2014/3/16

Y1 - 2014/3/16

N2 - The objective of this work was to study the effect of sample oil concentration and volume on hydroperoxide determination in n-3 PUFA-rich sardine-anchovy oil. Oil samples were stored at-20°C, 6 d, air-free (T1); and 20°C, 2 d in air (T2). Significant differences between storage conditions were observed on hydroperoxide concentration. High reproducibility was obtained even in sample concentrations as low as 5 mg oil/mL of propan-1-ol. A high correlation was observed between the modified ferrous oxidation-xylenol orange method (FOX2) and AOAC peroxide value analysis methods, although FOX2 was faster, more sensitive, and precise when analyzing small hydroperoxide concentrations.

AB - The objective of this work was to study the effect of sample oil concentration and volume on hydroperoxide determination in n-3 PUFA-rich sardine-anchovy oil. Oil samples were stored at-20°C, 6 d, air-free (T1); and 20°C, 2 d in air (T2). Significant differences between storage conditions were observed on hydroperoxide concentration. High reproducibility was obtained even in sample concentrations as low as 5 mg oil/mL of propan-1-ol. A high correlation was observed between the modified ferrous oxidation-xylenol orange method (FOX2) and AOAC peroxide value analysis methods, although FOX2 was faster, more sensitive, and precise when analyzing small hydroperoxide concentrations.

KW - Fish oil

KW - Hydroperoxides

KW - Lipid oxidation

KW - n-3 Fatty acids

UR - http://www.scopus.com/inward/record.url?scp=84888334942&partnerID=8YFLogxK

U2 - 10.1080/10942912.2012.660719

DO - 10.1080/10942912.2012.660719

M3 - Artículo

SN - 1094-2912

VL - 17

SP - 650

EP - 660

JO - International Journal of Food Properties

JF - International Journal of Food Properties

IS - 3

ER -

Effect of sample solubilized aliquot volume and concentration on hydroperoxide detection in PUFA-rich fish oil

Abstract

Keywords

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