TY - JOUR
T1 - Effect of moderate exercise on IgA levels and lymphocyte count in mouse intestine
AU - Viloria, María
AU - Lara-Padilla, Eleazar
AU - Campos-Rodríguez, Rafael
AU - Jarillo-Luna, Adriana
AU - Reyna-Garfias, Humberto
AU - López-Sánchez, Pedro
AU - Rivera-Aguilar, Víctor
AU - Salas-Casas, Andrés
AU - Berral De La Rosa, Francisco J.
AU - García-Latorre, Ethel
N1 - Funding Information:
We thank Bruce Allan Larsen for reviewing the use of English in this manuscript. This work was supported in part by grants from SEPI-IPN and from Universidad Pablo de Olavide, Spain. M. Viloria received a scholarship from Conacyt for graduate studies # 207126. E. García-Latorre, R. Campos-Rodríguez, E. Lara-Padilla, P. López-Sánchez, A. Salas-Casas and A. Jarillo-Luna are fellows of COFAA and EDI-IPN.
PY - 2011
Y1 - 2011
N2 - The aim of the present study was to determine the effect of moderate exercise on the production and secretion of IgA in mouse duodenum, on lymphocyte levels in the lamina propria, and on gene expression encoding for cytokines that regulate the synthesis of α-chain of IgA and the expression of pIgR in the lamina propria. Two groups of young Balb/c mice were fed ad libitum, one sedentary and the other with an exercise program (swimming) for 16 weeks. IgA levels in the duodenum were quantified by ELISA; the number of IgA containing cells as well as B cells, CD4 + and CD8 + T cells in the duodenal mucosa was determined by immunohistochemistry; gene expression was analyzed by real-time PCR, and the expression of proteins by Western blotting. Because of physical training, in the duodenum there was a decrease in the number of IgA producing cells, but an increase in the levels of IgA. Additionally, exercise increased the expression of the genes encoding for IL-4, IL-6, IL-10, TNF-α and TGF β, cytokines that regulate the synthesis of IgA and pIgR, the inflammatory response, and the immune response in the intestine. Thus, the increased IgA found in the duodenal lumen is probably due to the increased production of IgA in the LP and the increased transport of the pIgA-pIgR complex across epithelial cells. Possibly the increased S-IgA levels in the bile also contribute to the change in IgA levels.
AB - The aim of the present study was to determine the effect of moderate exercise on the production and secretion of IgA in mouse duodenum, on lymphocyte levels in the lamina propria, and on gene expression encoding for cytokines that regulate the synthesis of α-chain of IgA and the expression of pIgR in the lamina propria. Two groups of young Balb/c mice were fed ad libitum, one sedentary and the other with an exercise program (swimming) for 16 weeks. IgA levels in the duodenum were quantified by ELISA; the number of IgA containing cells as well as B cells, CD4 + and CD8 + T cells in the duodenal mucosa was determined by immunohistochemistry; gene expression was analyzed by real-time PCR, and the expression of proteins by Western blotting. Because of physical training, in the duodenum there was a decrease in the number of IgA producing cells, but an increase in the levels of IgA. Additionally, exercise increased the expression of the genes encoding for IL-4, IL-6, IL-10, TNF-α and TGF β, cytokines that regulate the synthesis of IgA and pIgR, the inflammatory response, and the immune response in the intestine. Thus, the increased IgA found in the duodenal lumen is probably due to the increased production of IgA in the LP and the increased transport of the pIgA-pIgR complex across epithelial cells. Possibly the increased S-IgA levels in the bile also contribute to the change in IgA levels.
KW - Cytokines
KW - Exercise
KW - Intestinal IgA
KW - Lymphocytes
KW - Physical training
KW - pIgR
UR - http://www.scopus.com/inward/record.url?scp=79959602478&partnerID=8YFLogxK
U2 - 10.3109/08820139.2011.575425
DO - 10.3109/08820139.2011.575425
M3 - Artículo
C2 - 21554181
SN - 0882-0139
VL - 40
SP - 640
EP - 656
JO - Immunological Investigations
JF - Immunological Investigations
IS - 6
ER -