Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture

E. Molina Grima; J. A. Sánchez Pérez; F. García Camacho; J. M. Fernández Sevilla; F. G. Acién Fernández

doi:10.1007/BF00166076

Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture

E. Molina Grima, J. A. Sánchez Pérez, F. García Camacho, J. M. Fernández Sevilla, F. G. Acién Fernández

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Abstract

An isolate of Isochrysis galbana rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been grown as a chemostat culture at 20° C and pH 8.00 controlled by CO₂ injection. From a low dilution rate (D) of 0.0024 h^-1 to 0.0377 h^-1, close to maximum growth, a decrease in EPA content from 5.21% dry weight (d.w.) to 2.80% d.w. was observed, although the percentage of EPA in the total fatty acids increased. Lipids were fractionated, EPA being the major fatty acid found in the glycolipid fraction, whereas in the neutral lipid fraction were mainly palmitic and palmitoleic acids. At the same time, the biomass concentration also decreased from 1015 mg·l^-1 to 202 mg·l^-1 over the range of Ds mentioned. Nonetheless, EPA productivity had a maximum value of 15.26 mg·l^-1·day^-1 at D=0.0208 h^-1.

Original language	English
Pages (from-to)	23-27
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	41
Issue number	1
DOIs	https://doi.org/10.1007/BF00166076
State	Published - Mar 1994
Externally published	Yes

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title = "Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture",

abstract = "An isolate of Isochrysis galbana rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been grown as a chemostat culture at 20° C and pH 8.00 controlled by CO2 injection. From a low dilution rate (D) of 0.0024 h-1 to 0.0377 h-1, close to maximum growth, a decrease in EPA content from 5.21% dry weight (d.w.) to 2.80% d.w. was observed, although the percentage of EPA in the total fatty acids increased. Lipids were fractionated, EPA being the major fatty acid found in the glycolipid fraction, whereas in the neutral lipid fraction were mainly palmitic and palmitoleic acids. At the same time, the biomass concentration also decreased from 1015 mg·l-1 to 202 mg·l-1 over the range of Ds mentioned. Nonetheless, EPA productivity had a maximum value of 15.26 mg·l-1·day-1 at D=0.0208 h-1.",

author = "{Molina Grima}, E. and {S{\'a}nchez P{\'e}rez}, {J. A.} and {Garc{\'i}a Camacho}, F. and {Fern{\'a}ndez Sevilla}, {J. M.} and {Aci{\'e}n Fern{\'a}ndez}, {F. G.}",

year = "1994",

month = mar,

doi = "10.1007/BF00166076",

language = "Ingl{\'e}s",

volume = "41",

pages = "23--27",

journal = "Applied Microbiology and Biotechnology",

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T1 - Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture

AU - Molina Grima, E.

AU - Sánchez Pérez, J. A.

AU - García Camacho, F.

AU - Fernández Sevilla, J. M.

AU - Acién Fernández, F. G.

PY - 1994/3

Y1 - 1994/3

N2 - An isolate of Isochrysis galbana rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been grown as a chemostat culture at 20° C and pH 8.00 controlled by CO2 injection. From a low dilution rate (D) of 0.0024 h-1 to 0.0377 h-1, close to maximum growth, a decrease in EPA content from 5.21% dry weight (d.w.) to 2.80% d.w. was observed, although the percentage of EPA in the total fatty acids increased. Lipids were fractionated, EPA being the major fatty acid found in the glycolipid fraction, whereas in the neutral lipid fraction were mainly palmitic and palmitoleic acids. At the same time, the biomass concentration also decreased from 1015 mg·l-1 to 202 mg·l-1 over the range of Ds mentioned. Nonetheless, EPA productivity had a maximum value of 15.26 mg·l-1·day-1 at D=0.0208 h-1.

AB - An isolate of Isochrysis galbana rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been grown as a chemostat culture at 20° C and pH 8.00 controlled by CO2 injection. From a low dilution rate (D) of 0.0024 h-1 to 0.0377 h-1, close to maximum growth, a decrease in EPA content from 5.21% dry weight (d.w.) to 2.80% d.w. was observed, although the percentage of EPA in the total fatty acids increased. Lipids were fractionated, EPA being the major fatty acid found in the glycolipid fraction, whereas in the neutral lipid fraction were mainly palmitic and palmitoleic acids. At the same time, the biomass concentration also decreased from 1015 mg·l-1 to 202 mg·l-1 over the range of Ds mentioned. Nonetheless, EPA productivity had a maximum value of 15.26 mg·l-1·day-1 at D=0.0208 h-1.

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JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 1

ER -

Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture

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