TY - JOUR
T1 - Effect of constitutive miR164 expression on plant morphology and fruit development in Arabidopsis and tomato
AU - Rosas Cárdenas, Flor De Fátima
AU - Suárez, Yolanda Ruiz
AU - Rangel, Rosa María Cano
AU - Garcia, Valentín Luna
AU - Aguilera, Karla Lorena González
AU - Martínez, Nayelli Marsch
AU - De Folter, Stefan
N1 - Publisher Copyright:
© 2017 by the authors.
PY - 2017/7/15
Y1 - 2017/7/15
N2 - Several miRNAs are conserved in different plant families, but their abundance and target genes vary between species, organs, and stages of development. Target genes of miRNAs are mostly transcription factors, involved in the control of many plant developmental processes, including fruit development. MiR164 is a conserved miRNA, highly expressed in fruits, and is validated to target a subset of genes of the NAC-domain transcription factor gene family. The objective of this work was to analyze the phenotypic effects of the constitutive expression of miR164 during the life cycle of Arabidopsis and tomato. MiR164 overexpression (164-OE) lines for Arabidopsis and tomato were generated and analyzed during plant development. The constitutive miR164 expression showed that miR164 affected the morphology of Arabidopsis and tomato, and it affected the transition from the vegetative to the reproductive phase in Arabidopsis. Moreover, the miR164 overexpression affected the time required for each developmental stage of tomato fruit. These results suggest that miR164 plays general and specific roles during development in Arabidopsis and tomato, including fruit development, which could be exploited for the improvement of traits of agronomic interest in important species.
AB - Several miRNAs are conserved in different plant families, but their abundance and target genes vary between species, organs, and stages of development. Target genes of miRNAs are mostly transcription factors, involved in the control of many plant developmental processes, including fruit development. MiR164 is a conserved miRNA, highly expressed in fruits, and is validated to target a subset of genes of the NAC-domain transcription factor gene family. The objective of this work was to analyze the phenotypic effects of the constitutive expression of miR164 during the life cycle of Arabidopsis and tomato. MiR164 overexpression (164-OE) lines for Arabidopsis and tomato were generated and analyzed during plant development. The constitutive miR164 expression showed that miR164 affected the morphology of Arabidopsis and tomato, and it affected the transition from the vegetative to the reproductive phase in Arabidopsis. Moreover, the miR164 overexpression affected the time required for each developmental stage of tomato fruit. These results suggest that miR164 plays general and specific roles during development in Arabidopsis and tomato, including fruit development, which could be exploited for the improvement of traits of agronomic interest in important species.
KW - Arabidopsis
KW - Fruit development
KW - MiR164
KW - MiRNA
KW - Overexpression miRNA
KW - Tomato
UR - http://www.scopus.com/inward/record.url?scp=85028702345&partnerID=8YFLogxK
U2 - 10.3390/agronomy7030048
DO - 10.3390/agronomy7030048
M3 - Artículo
SN - 2073-4395
VL - 7
JO - Agronomy
JF - Agronomy
IS - 3
M1 - 48
ER -