TY - JOUR
T1 - Effect of chitosan type on protein and water recovery efficiency from surimi wash water treated with chitosan-alginate complexes
AU - Wibowo, Singgih
AU - Velazquez, Gonzalo
AU - Savant, Vivek
AU - Torres, J. Antonio
N1 - Funding Information:
The authors would like to thank Dr. Il-Shik Moon (26), Department of Chemical Engineering, College of Engineering, Sun Chon National University for providing experimental chitosan samples. This work was supported by Grant No. NA 16RG 1039 (Project No. R/SF-29) from the National Oceanic and Atmospheric Administration to the Oregon State University Sea Grant College Program, and from appropriations made by the Oregon State Legislature. Financial assistance was provided to Author Wibowo by the Participatory Development of Agricultural Technology Project (PAATP, ADB Loan No. 1526 INO) of the Agency for Agricultural Research and Development (AARD), the Government of Indonesia. Thanks also to Dr. Christine Pastorek in the OSU Chem. Dept. for assistance with FTIR analysis and Dr. Alberto Herrera-Gómez (CINVESTAV-Querétaro, Mexico) for the use of the deconvolution software. A free copy of AAnalyzer (Register Number 03-1999-051710412300, Mexico) can be requested at alberto@ciateq.mx or downloaded from http://qro.cinvestav.mx/~aanalyzer .
PY - 2007/2
Y1 - 2007/2
N2 - Previous research has shown that soluble protein recovery by chitosan (Chi) complexes with polyanions such as alginate (Alg) is more effective than using chitosan alone. In this study, Chi-Alg complexes were used to recover soluble proteins from surimi wash water (SWW) slightly acidified to pH 6. Six Chi samples differing in molecular weight (MW) and degree of deacetylation (DD) were used at 20, 40 and 100 mg/L SWW Chi-Alg complexes prepared with a Chi:Alg mixing ratio previously optimized (MR = 0.2). FTIR analysis of the solids recovered revealed the three characteristic amide bands observed in the same region for untreated SWW confirming protein adsorption by Chi-Alg. The superior effectiveness of Chi complexes was confirmed but differences among chitosan types could not be correlated to MW and DD. Experimental Chi samples with 94%, 93%, 75% and 93% DD and 22, 47, 225 and 3404 × 103 Da, respectively, showed 73-76% protein adsorption while a commercial chitosan sample with 84% DD and 3832 × 103 Da had 74-83% protein adsorption. An experimental chitosan, SY-1000 with 94% DD and 1.5 × 106 Da, showed the highest protein adsorption (79-86%) and turbidity reduction (85-92%) when used at 20 mg/L SWW.
AB - Previous research has shown that soluble protein recovery by chitosan (Chi) complexes with polyanions such as alginate (Alg) is more effective than using chitosan alone. In this study, Chi-Alg complexes were used to recover soluble proteins from surimi wash water (SWW) slightly acidified to pH 6. Six Chi samples differing in molecular weight (MW) and degree of deacetylation (DD) were used at 20, 40 and 100 mg/L SWW Chi-Alg complexes prepared with a Chi:Alg mixing ratio previously optimized (MR = 0.2). FTIR analysis of the solids recovered revealed the three characteristic amide bands observed in the same region for untreated SWW confirming protein adsorption by Chi-Alg. The superior effectiveness of Chi complexes was confirmed but differences among chitosan types could not be correlated to MW and DD. Experimental Chi samples with 94%, 93%, 75% and 93% DD and 22, 47, 225 and 3404 × 103 Da, respectively, showed 73-76% protein adsorption while a commercial chitosan sample with 84% DD and 3832 × 103 Da had 74-83% protein adsorption. An experimental chitosan, SY-1000 with 94% DD and 1.5 × 106 Da, showed the highest protein adsorption (79-86%) and turbidity reduction (85-92%) when used at 20 mg/L SWW.
KW - Alginate
KW - Chitosan
KW - Flocculation
KW - Surimi
KW - Turbidity
UR - http://www.scopus.com/inward/record.url?scp=33748567825&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2006.02.014
DO - 10.1016/j.biortech.2006.02.014
M3 - Artículo
SN - 0960-8524
VL - 98
SP - 539
EP - 545
JO - Bioresource Technology
JF - Bioresource Technology
IS - 3
ER -