Abstract
Bioaugmented-assisted remediation of a hydrocarbon-polluted soil was performed in sealed serum bottles using a native and well adapted mixed culture consisting on S. liquefaciens and B. coagulans. The soil for this study was acidic (pH 3.8) with a total petroleum hydrocarbon (TPH) concentration of 220,000 mg/kg soil and a native microbial population of 2.0×105 CFU/g soil. This study examines a biological treatment to clean up petroleum-polluted soil using a mixed culture composed by S. liquefaciens and B. coagulans. C/N/P ratio, moisture and pH were adjusted to appropriate values to assist the biodegradation process. Results demonstrated that heterotrophic activity in microcosms was higher (1.9 mg/g) in bioaugmented microcosms compared with non-bioaugmented (1.3 mg/g). During all the incubation period only three strains were cultivated S. liquefaciens, B. coagulans and a non-identified yellow bacteria. The TPHs removal increased 12% in bioaugmented microcosms at the end of 74 days of treatment. In the case of aromatic and asphaltenic fractions the removals increased 12 and 10% respectively. During the first 35 days of incubation, TPHs removal correlates with the exponential phase of microbial growth. Our results showed that bioaugmented-assisted remediation of hydrocarbon-polluted soils in highly polluted and weathered sites are feasible.
Original language | English |
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Title of host publication | Contaminated Soils, Sediments and Water |
Publisher | Springer US |
Pages | 1-11 |
Number of pages | 11 |
Volume | 9 |
ISBN (Print) | 038723036X, 9780387230368 |
DOIs | |
State | Published - 2005 |
Externally published | Yes |
Keywords
- aromatic
- asphaltenic
- bioaugmentation
- removal