Effect of bacteria augmentation on aromatic and asphaltenic fraction removal in solid culture

E. Cervantes-Gonzalez, L. I. Rojas-Avelizapa, R. Cruz-Camarillo, N. G. Rojas-Avelizapa

    Research output: Chapter in Book/Report/Conference proceedingChapter

    1 Scopus citations


    Bioaugmented-assisted remediation of a hydrocarbon-polluted soil was performed in sealed serum bottles using a native and well adapted mixed culture consisting on S. liquefaciens and B. coagulans. The soil for this study was acidic (pH 3.8) with a total petroleum hydrocarbon (TPH) concentration of 220,000 mg/kg soil and a native microbial population of 2.0×105 CFU/g soil. This study examines a biological treatment to clean up petroleum-polluted soil using a mixed culture composed by S. liquefaciens and B. coagulans. C/N/P ratio, moisture and pH were adjusted to appropriate values to assist the biodegradation process. Results demonstrated that heterotrophic activity in microcosms was higher (1.9 mg/g) in bioaugmented microcosms compared with non-bioaugmented (1.3 mg/g). During all the incubation period only three strains were cultivated S. liquefaciens, B. coagulans and a non-identified yellow bacteria. The TPHs removal increased 12% in bioaugmented microcosms at the end of 74 days of treatment. In the case of aromatic and asphaltenic fractions the removals increased 12 and 10% respectively. During the first 35 days of incubation, TPHs removal correlates with the exponential phase of microbial growth. Our results showed that bioaugmented-assisted remediation of hydrocarbon-polluted soils in highly polluted and weathered sites are feasible. © 2005 Springer Science + Business Media, Inc.
    Original languageAmerican English
    Title of host publicationContaminated Soils, Sediments and Water
    Number of pages1
    ISBN (Electronic)038723036X, 9780387230368
    StatePublished - 1 Dec 2005


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