TY - JOUR
T1 - Effect of 6-nonadecyl salicylic acid and its methyl ester on the induction of micronuclei in polychromatic erythrocytes in mouse peripheral blood
AU - Acevedo, Hortensia Rosas
AU - Rojas, Maritere Domínguez
AU - Arceo, Sandra Díaz Barriga
AU - Soto Hernández, Marcos
AU - Martínez Vázquez, Mariano
AU - Terrazas, Teresa
AU - del Toro, Gustavo Valencia
N1 - Funding Information:
One of the authors (Hortensia Rosas Acevedo) wishes to thank the Consejo Nacional de Ciencia y Tecnología (CONACYT) for a grant for PhD studies.
PY - 2006/10/10
Y1 - 2006/10/10
N2 - The bark of Amphipterygium adstringens is widely used in the traditional Mexican medicine for treating ailments such as gastric ulcers, gastritis and stomach cancer. The 6-nonadecyl salicylic acid (anacardic acid) was isolated from the bark of this species. In previous papers have been informed that the anacardic acids possess anti-tumour, antimicrobial, antiacne, antibacterial and many others medicinal properties. Now we describe cytotoxic and genotoxic effects of this compound and its methyl ester. The cytotoxic and genotoxic effects of 6-nonadecyl salicylic acid (6NDSA) and its methyl ester (ME6NDSA) on CD1 male mice were determined with micronucleus assay at 24, 48 and 72 h after oral administration of doses of 0.75, 2.5, 5.0 and 10.0 mg/kg. Peripheral blood samples were drawn from the caudal vein and analyzed by Giemsa-stained technique. The results obtained showed that the ratios of polychromatic erythrocytes (PCE) to normochromatic erythrocytes (NCE) in mice treated with 10 mg/kg of 6NDSA were statistically lower after 24 h compared with its negative control animals, and that after 72 h, PCE/NCE ratios were reduced in animals treated with 6NDSA at all tested dose levels. The methyl ester ME6NDSA showed no such cytotoxic activity. Neither of the test compounds increased the frequency of micronucleated polychromatic erythrocytes from which it appears that administration of 6NDSA and ME6NDSA may not lead to chromosome damage at the evaluated doses.
AB - The bark of Amphipterygium adstringens is widely used in the traditional Mexican medicine for treating ailments such as gastric ulcers, gastritis and stomach cancer. The 6-nonadecyl salicylic acid (anacardic acid) was isolated from the bark of this species. In previous papers have been informed that the anacardic acids possess anti-tumour, antimicrobial, antiacne, antibacterial and many others medicinal properties. Now we describe cytotoxic and genotoxic effects of this compound and its methyl ester. The cytotoxic and genotoxic effects of 6-nonadecyl salicylic acid (6NDSA) and its methyl ester (ME6NDSA) on CD1 male mice were determined with micronucleus assay at 24, 48 and 72 h after oral administration of doses of 0.75, 2.5, 5.0 and 10.0 mg/kg. Peripheral blood samples were drawn from the caudal vein and analyzed by Giemsa-stained technique. The results obtained showed that the ratios of polychromatic erythrocytes (PCE) to normochromatic erythrocytes (NCE) in mice treated with 10 mg/kg of 6NDSA were statistically lower after 24 h compared with its negative control animals, and that after 72 h, PCE/NCE ratios were reduced in animals treated with 6NDSA at all tested dose levels. The methyl ester ME6NDSA showed no such cytotoxic activity. Neither of the test compounds increased the frequency of micronucleated polychromatic erythrocytes from which it appears that administration of 6NDSA and ME6NDSA may not lead to chromosome damage at the evaluated doses.
KW - Amphipterygium adstringens
KW - Anacardic acids
KW - Cuachalalate
KW - Cytotoxicity
KW - Genotoxicity
UR - http://www.scopus.com/inward/record.url?scp=33748439124&partnerID=8YFLogxK
U2 - 10.1016/j.mrgentox.2006.06.002
DO - 10.1016/j.mrgentox.2006.06.002
M3 - Artículo
C2 - 16857418
SN - 1383-5718
VL - 609
SP - 43
EP - 46
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 1
ER -