Abstract
Eysenhardtia polystachya is used widely in tradicional medicine to treat the urolithiasis. In this study was evaluated the inhibitory effect of 7-hydroxy-2′,4′,5′-trime-thoxyisoflavone (1) and 7-hydroxy-4′-ethoxyisoflavone (2) isolated from bark of E. polystachya on aggregation calcium oxalate crystals in two different assay with a non-crystal-seed system. The measurement of inhibitory activity on crystal aggregation of the isoflavones isolated from Eysenhardti polystachya performed on simulated, recurrent stone-formers and normal urine. The inhibitory effect of flavonoid on calcium oxalate crystal aggregation was measured with an aggregometer, and in the assay of non-crystal-seed system the calcium was measured using the colorimetrically method with cresophthalein complexone. Both isoflavones showed a marked inhibitory activity on crystal aggregation on urine non-stone-formers, whereas in urine from recurrent stone-formers and simulated orine showed less inhibitory activity compared to normal urine. The efect was compared with addition of heparin, sodium salt. Also the effect of the isoflavones (1) and (2) on crystal growth of calcium oxalate (COM) at conditions of constant solution supersaturation was determined. The isolated isoflavones 7-hydroxy-2′,4′,5′-trimethoxyisoflavone and 7-hydroxy-4′-ethoxyisoflavone from E. polystacha work as inhibitors in the formation and crystal growth of calcium oxalate, reduced the degree of aggregation and the size of particles precipitated.
| Translated title of the contribution | Effect of isoflavones from Eysenhardtia polystachya as inhibitors of calcium oxalate crystal aggregation |
|---|---|
| Original language | Spanish |
| Pages (from-to) | 20-26 |
| Number of pages | 7 |
| Journal | Revista Mexicana de Ciencias Farmaceuticas |
| Volume | 35 |
| Issue number | 3 |
| State | Published - Jul 2004 |
| Externally published | Yes |