TY - JOUR
T1 - Early development and life cycle of Contracaecum multipapillatum from a brown pelican Pelecanus occidentalis in the Gulf of California, Mexico
AU - Valles-Vega, Isabel
AU - Molina-Fernández, Dolores
AU - Benítez, Rocío
AU - Hernández-Trujillo, Sergio
AU - Adroher, Francisco Javier
N1 - Publisher Copyright:
© Inter-Research 2017.
PY - 2017/8/9
Y1 - 2017/8/9
N2 - The initial developmental stages of Contracaecum multipapillatum (von Drasche, 1882) Lucker, 1941 sensu lato were studied using eggs obtained from the uteri of female nematodes (genetically identified) found in a brown pelican Pelecanus occidentalis from Bahía de La Paz (Gulf of California, Mexico). Optical microscopy revealed a smooth or slightly rough surface to the eggs. Egg dimensions were approximately 53 × 43 μm, although after the larvae had developed inside, egg size increased to 66 × 55 μm. Hatching and survival of the larvae were greater at 15°C than at 24°C, and increased salinity resulted in a slight increase in hatching but seemed to reduce survival at 24°C, but not at 15°C. The recently hatched larvae measured 261 × 16 μm within their sheath. When placed in culture medium, the larvae grew within their sheath, and a small percentage (∼2%) exsheathed completely (314 × 19 μm). The larvae continued to grow and develop once they had exsheathed, attaining mean dimensions of 333 × 22 μm. Although they did not moult during culture, optical microscopy revealed a morphology typical of third-stage larvae. Finally, the genetic identity between the larval parasites collected from mullet Mugil curema and adult female parasites collected from the brown pelican suggests a life cycle of C. multipapillatum in which the mullet are involved as intermediate/paratenic hosts and the brown pelicans as final hosts in the geographical area of Bahía de La Paz.
AB - The initial developmental stages of Contracaecum multipapillatum (von Drasche, 1882) Lucker, 1941 sensu lato were studied using eggs obtained from the uteri of female nematodes (genetically identified) found in a brown pelican Pelecanus occidentalis from Bahía de La Paz (Gulf of California, Mexico). Optical microscopy revealed a smooth or slightly rough surface to the eggs. Egg dimensions were approximately 53 × 43 μm, although after the larvae had developed inside, egg size increased to 66 × 55 μm. Hatching and survival of the larvae were greater at 15°C than at 24°C, and increased salinity resulted in a slight increase in hatching but seemed to reduce survival at 24°C, but not at 15°C. The recently hatched larvae measured 261 × 16 μm within their sheath. When placed in culture medium, the larvae grew within their sheath, and a small percentage (∼2%) exsheathed completely (314 × 19 μm). The larvae continued to grow and develop once they had exsheathed, attaining mean dimensions of 333 × 22 μm. Although they did not moult during culture, optical microscopy revealed a morphology typical of third-stage larvae. Finally, the genetic identity between the larval parasites collected from mullet Mugil curema and adult female parasites collected from the brown pelican suggests a life cycle of C. multipapillatum in which the mullet are involved as intermediate/paratenic hosts and the brown pelicans as final hosts in the geographical area of Bahía de La Paz.
KW - Anisakidae
KW - Aquatic life cycle
KW - Brown pelican
KW - Contracaecum multipapillatum
KW - Development
KW - Fish parasite
KW - Genetic Identification
KW - Gulf of California
KW - Mullet
KW - Nematoda
UR - http://www.scopus.com/inward/record.url?scp=85027351298&partnerID=8YFLogxK
U2 - 10.3354/dao03147
DO - 10.3354/dao03147
M3 - Artículo
C2 - 28792415
SN - 0177-5103
VL - 125
SP - 167
EP - 178
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
IS - 3
ER -