TY - JOUR
T1 - Draft genome of five Cupriavidus plantarum strains
T2 - agave, maize and sorghum plant-associated bacteria with resistance to metals
AU - Arroyo-Herrera, Ivan
AU - Rojas-Rojas, Fernando Uriel
AU - Lozano-Cervantes, Karla Daniela
AU - Larios-Serrato, Violeta
AU - Vásquez-Murrieta, María Soledad
AU - Whtiman, William B.
AU - Ibarra, J. Antonio
AU - Estrada-de los Santos, Paulina
N1 - Publisher Copyright:
© 2020, King Abdulaziz City for Science and Technology.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Five strains of Cupriavidus plantarum, a metal-resistant, plant-associated bacterium, were selected for genome sequencing through the Genomic Encyclopedia of Bacteria and Archaea (GEBA) Phase IV project at the Joint Genome Institute (JGI) of the U.S. Department of Energy (DOE). The genome of the strains was in the size range of 6.2–6.4 Mbp and encoded 5605–5834 proteins; 16.9–23.7% of these genes could not be assigned to a COG-associated functional category. The G + C content was 65.83–65.99%, and the genomes encoded 59–67 stable RNAs. The strains were resistant in vitro to arsenite, arsenate, cobalt, chromium, copper, nickel and zinc, and their genomes possessed the resistance genes for these metals. The genomes also encoded the biosynthesis of potential antimicrobial compounds, such as terpenes, phosphonates, bacteriocins, betalactones, nonribosomal peptides, phenazine and siderophores, as well as the biosynthesis of cellulose and enzymes such as chitinase and trehalase. The average nucleotide identity (ANI) and DNA-DNA in silico hybridization of the genomes confirmed that C. plantarum is a single species. Moreover, the strains cluster within a single group upon multilocus sequence analyses with eight genes and a phylogenomic analyses. Noteworthy, the ability of the species to tolerate high concentrations of different metals might prove useful for bioremediation of naturally contaminated environments.
AB - Five strains of Cupriavidus plantarum, a metal-resistant, plant-associated bacterium, were selected for genome sequencing through the Genomic Encyclopedia of Bacteria and Archaea (GEBA) Phase IV project at the Joint Genome Institute (JGI) of the U.S. Department of Energy (DOE). The genome of the strains was in the size range of 6.2–6.4 Mbp and encoded 5605–5834 proteins; 16.9–23.7% of these genes could not be assigned to a COG-associated functional category. The G + C content was 65.83–65.99%, and the genomes encoded 59–67 stable RNAs. The strains were resistant in vitro to arsenite, arsenate, cobalt, chromium, copper, nickel and zinc, and their genomes possessed the resistance genes for these metals. The genomes also encoded the biosynthesis of potential antimicrobial compounds, such as terpenes, phosphonates, bacteriocins, betalactones, nonribosomal peptides, phenazine and siderophores, as well as the biosynthesis of cellulose and enzymes such as chitinase and trehalase. The average nucleotide identity (ANI) and DNA-DNA in silico hybridization of the genomes confirmed that C. plantarum is a single species. Moreover, the strains cluster within a single group upon multilocus sequence analyses with eight genes and a phylogenomic analyses. Noteworthy, the ability of the species to tolerate high concentrations of different metals might prove useful for bioremediation of naturally contaminated environments.
KW - Cupriavidus
KW - Metal resistance
KW - PGP bacteria
KW - Plant-associated bacteria
KW - Rhizobacteria
UR - http://www.scopus.com/inward/record.url?scp=85084270435&partnerID=8YFLogxK
U2 - 10.1007/s13205-020-02210-8
DO - 10.1007/s13205-020-02210-8
M3 - Artículo
AN - SCOPUS:85084270435
SN - 2190-572X
VL - 10
JO - 3 Biotech
JF - 3 Biotech
IS - 6
M1 - 242
ER -