TY - JOUR
T1 - Double-stranded RNA against white spot syndrome virus (WSSV) vp28 or vp26 reduced susceptibility of Litopenaeus vannamei to WSSV, and survivors exhibited decreased susceptibility in subsequent re-infections
AU - Mejía-Ruíz, Claudio Humberto
AU - Vega-Peña, Sergio
AU - Alvarez-Ruiz, Píndaro
AU - Escobedo-Bonilla, César Marcial
PY - 2011/5
Y1 - 2011/5
N2 - The antiviral effect of vp28 or vp26 double-stranded (ds) RNA upon single or consecutive white spot syndrome virus (WSSV) intramuscular challenges with a high infectious dose was evaluated. The vp28 dsRNA showed the highest protection both in single (LT50=145h at 10d and 98h at 20d post treatment [dpt]) or consecutive (LT50=765h) WSSV challenges compared to vp26 dsRNA (LT50=126h at 10 d and 57h at 20dpt vs. consecutive challenge LT50=751h). Single WSSV challenges showed that animals treated with vp28 or vp26 dsRNA gradually lost the antiviral effect as virus challenge occurred at 10dpt (cumulative mortality 63% vs. 80%, respectively) or 20dpt (87% vs. 100%, respectively). In contrast, animals treated with vp28 or vp26 dsRNA and consecutively challenged with WSSV showed and extended lower susceptibility to WSSV. All dead animals were WSSV-positive by one-step PCR, whereas all surviving shrimp from single or continuous challenges were WSSV-negative as determined by reverse transcription (RT)-PCR. In conclusion, shrimp treated with a single administration of vp28 or vp26 dsRNA and consecutively challenged with WSSV showed a stronger and longer antiviral response than shrimp exposed once to WSSV at 10 or 20dpt.
AB - The antiviral effect of vp28 or vp26 double-stranded (ds) RNA upon single or consecutive white spot syndrome virus (WSSV) intramuscular challenges with a high infectious dose was evaluated. The vp28 dsRNA showed the highest protection both in single (LT50=145h at 10d and 98h at 20d post treatment [dpt]) or consecutive (LT50=765h) WSSV challenges compared to vp26 dsRNA (LT50=126h at 10 d and 57h at 20dpt vs. consecutive challenge LT50=751h). Single WSSV challenges showed that animals treated with vp28 or vp26 dsRNA gradually lost the antiviral effect as virus challenge occurred at 10dpt (cumulative mortality 63% vs. 80%, respectively) or 20dpt (87% vs. 100%, respectively). In contrast, animals treated with vp28 or vp26 dsRNA and consecutively challenged with WSSV showed and extended lower susceptibility to WSSV. All dead animals were WSSV-positive by one-step PCR, whereas all surviving shrimp from single or continuous challenges were WSSV-negative as determined by reverse transcription (RT)-PCR. In conclusion, shrimp treated with a single administration of vp28 or vp26 dsRNA and consecutively challenged with WSSV showed a stronger and longer antiviral response than shrimp exposed once to WSSV at 10 or 20dpt.
KW - Consecutive WSSV inoculations
KW - DsRNA
KW - Extended susceptibility reduction
KW - Inhibition of virus replication
KW - Litopenaeus vannamei
UR - http://www.scopus.com/inward/record.url?scp=79953772696&partnerID=8YFLogxK
U2 - 10.1016/j.jip.2011.02.002
DO - 10.1016/j.jip.2011.02.002
M3 - Artículo
C2 - 21345339
SN - 0022-2011
VL - 107
SP - 65
EP - 68
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 1
ER -