TY - JOUR
T1 - DNA purification using a novel γ-Fe2O3/PEDOT hybrid nanocomposite
AU - da Silva, Romário J.
AU - Pedro, Graciela C.
AU - Gorza, Filipe D.S.
AU - Maciel, Bruna G.
AU - Ratkovski, Gabriela P.
AU - Mojica-Sánchez, Lizeth Carolina
AU - Medina-Llamas, Juan C.
AU - Chávez-Guajardo, Alicia E.
AU - de Melo, Celso P.
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/9/15
Y1 - 2021/9/15
N2 - We report the synthesis and characterization of a new hybrid magnetic composite formed by the enveloping of magnetic iron oxide nanoparticles (γ-NP) with chains of the conductive polymer PEDOT, and its use for the efficient separation of DNA molecules from complex biological samples, allowing the high yield separation of a pure and high-quality DNA fraction. The successful formation of the γ-NP/PEDOT composite was confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy, UV visible spectroscopy (UV–Vis), and magnetic hysteresis loop measurements. The nanocomposites showed an excellent capacity of DNA adsorption (Qe ∼ 248 mg/g) in a model system consisting of salmon sperm DNA. When the γ-NP/PEDOT was used in protocols to extract the DNA from complex samples, the corresponding yield was in the range of 6.4 μg (blood) and 7.3 μg (bacteria), as evaluated quality by UV–Vis, PCR analysis, and electrophoresis assays. We also established that the captured DNA does not need to be detached from the nanocomposite for use as seeding material in PCR amplification experiments. These results and the simplicity of the protocols indicate that the γ-NP/PEDOT composite is a promising DNA absorbent, being competitive with the commercially available magnetic purification kits.
AB - We report the synthesis and characterization of a new hybrid magnetic composite formed by the enveloping of magnetic iron oxide nanoparticles (γ-NP) with chains of the conductive polymer PEDOT, and its use for the efficient separation of DNA molecules from complex biological samples, allowing the high yield separation of a pure and high-quality DNA fraction. The successful formation of the γ-NP/PEDOT composite was confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy, UV visible spectroscopy (UV–Vis), and magnetic hysteresis loop measurements. The nanocomposites showed an excellent capacity of DNA adsorption (Qe ∼ 248 mg/g) in a model system consisting of salmon sperm DNA. When the γ-NP/PEDOT was used in protocols to extract the DNA from complex samples, the corresponding yield was in the range of 6.4 μg (blood) and 7.3 μg (bacteria), as evaluated quality by UV–Vis, PCR analysis, and electrophoresis assays. We also established that the captured DNA does not need to be detached from the nanocomposite for use as seeding material in PCR amplification experiments. These results and the simplicity of the protocols indicate that the γ-NP/PEDOT composite is a promising DNA absorbent, being competitive with the commercially available magnetic purification kits.
KW - Conducting polymers
KW - DNA
KW - Escherichia coli
KW - Magnetic nanocomposite
KW - Magnetic purification
KW - Whole blood
UR - http://www.scopus.com/inward/record.url?scp=85109438050&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2021.338762
DO - 10.1016/j.aca.2021.338762
M3 - Artículo
C2 - 34482873
AN - SCOPUS:85109438050
SN - 0003-2670
VL - 1178
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
M1 - 338762
ER -