TY - JOUR
T1 - Dna barcoding of mosquitoes from the pantanos de centla biosphere reserve, southeastern mexico
AU - Ortega-Morales, Aldo I.
AU - Hernandez-Triana, Luis M.
AU - Chan-Chable, Rahuel J.
AU - Garza-Hernandez, Javier A.
AU - Gonzalez-Alvarez, Vicente H.
AU - Ruiz-Arrondo, Ignacio
AU - Nikolova, Nadya I.
AU - Martinez-Arce, Arely
AU - Fooks, Anthony R.
AU - Rodriguez-Perez, Mario A.
N1 - Publisher Copyright:
© 2021 American Mosquito Control Association. All rights reserved.
PY - 2021/12
Y1 - 2021/12
N2 - Accurate identification of mosquito species is essential to support programs that involve the study of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on morphological characteristics, due to the morphological similarities in different life stages and large numbers of some species that are members of morphologically similar species complexes. In the present study, the mosquitoes collected in the Pantanos de Centla Biosphere Reserve, southeastern Mexico, were evaluated using a combination of morphological and molecular approaches (mitochondrial cytochrome c oxidase subunit I [COI] DNA barcode). A total of 1,576 specimens of 10 genera and 35 species, mostly adult stages, were collected. A total of 225 COI DNA barcode sequences were analyzed; most species formed well-supported groups in the neighbor joining, maximum likelihood, and Bayesian inference trees. The intraspecific Kimura 2-parameter (K2P) genetic distance averaged 1.52%. An intraspecific K2P distance of 6.20% was observed in Anopheles crucians s.l., while a deep split was identified in Culex erraticus and Cx. conspirator. This study showed that COI DNA barcodes offer a reliable approach to support mosquito species identification in Mexico.
AB - Accurate identification of mosquito species is essential to support programs that involve the study of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on morphological characteristics, due to the morphological similarities in different life stages and large numbers of some species that are members of morphologically similar species complexes. In the present study, the mosquitoes collected in the Pantanos de Centla Biosphere Reserve, southeastern Mexico, were evaluated using a combination of morphological and molecular approaches (mitochondrial cytochrome c oxidase subunit I [COI] DNA barcode). A total of 1,576 specimens of 10 genera and 35 species, mostly adult stages, were collected. A total of 225 COI DNA barcode sequences were analyzed; most species formed well-supported groups in the neighbor joining, maximum likelihood, and Bayesian inference trees. The intraspecific Kimura 2-parameter (K2P) genetic distance averaged 1.52%. An intraspecific K2P distance of 6.20% was observed in Anopheles crucians s.l., while a deep split was identified in Culex erraticus and Cx. conspirator. This study showed that COI DNA barcodes offer a reliable approach to support mosquito species identification in Mexico.
KW - Culicidae
KW - Mexico
KW - Mitochondrial cytochrome c oxidase subunit I
KW - Tabasco State
UR - http://www.scopus.com/inward/record.url?scp=85121047797&partnerID=8YFLogxK
U2 - 10.2987/21-6967
DO - 10.2987/21-6967
M3 - Artículo
C2 - 34817614
AN - SCOPUS:85121047797
SN - 8756-971X
VL - 37
SP - 198
EP - 207
JO - Journal of the American Mosquito Control Association
JF - Journal of the American Mosquito Control Association
IS - 4
ER -
