TY - JOUR
T1 - Diversity and expression of P450 genes from Dendroctonus valens LeConte (Curculionidae: Scolytinae) in response to different kairomones
AU - López, María Fernanda
AU - Cano-Ramírez, Claudia
AU - Cesar-Ayala, Ana K.
AU - Ruiz, Enrico A.
AU - Zúñiga, Gerardo
N1 - Funding Information:
We thank Francisco Bonilla for his assistance in the field, María Cabañas and Guillermo Elizondo (CINVESTAV-IPN) for their assistance in the RT-qPCR, Kim Trovamala for her help in the experimental assays, and Rodolfo Montes for the elaboration of graphical abstract. Two anonymous reviewers made valuable suggestions. The project was funded by Comisión Nacional Forestal – Consejo Nacional de Ciencia y Tecnología ( CONAFOR-CONACYT 69539 ), CONACYT (181337/CB2012) and Secretaría de Investigación y Posgrado-IPN (SIP-20090576). This work was part of MFL's PhD. dissertation. MFL was Consejo Nacional de Ciencia y Tecnología and Programa Institucional de Formación de Investigadores del Instituto Politécnico Nacional (PIFI-IPN) fellow (175839, 202060). KCA was CONACyT (267462) and PIFI-IPN fellow.
PY - 2013/5
Y1 - 2013/5
N2 - Bark beetles (Curculionidae: Scolytinae) are major cause of woody plants death in the world. They colonize the stem and other parts of trees recognizing host-produced specific compounds (kairomones) and insect pheromones. Bark beetle's antennae and alimentary canal participate in the host selection identifying chemical compounds produced by trees and insects, and also in the metabolism and detoxification of these compounds. The red turpentine beetle (RTB), Dendroctonus valens LeConte, is an unaggressive species that colonize > 40 pine species (Pinaceae) in North and Central America. Several studies suggest that bark beetle cytochrome P450 enzymes are involved in monoterpene oxidation. In this study we identified by means of PCR, cloning, sequencing, and bioinformatic analysis, eleven full-length genes: five CYP4, four CYP6, and two CYP9 in the antennae and gut region of RTB, after stimulation with vapors of monoterpenes: (±)-α-pinene, (R)-(+)-α-pinene, (S)-(-)-β-pinene, (S)-(-)-α-pinene and (+)-3-carene; pine trees volatiles used by RTB as kairomones. The recovered cDNA of these genes vary from 1.5 kb to 1.8 kb and the open frame encodes from 496 to 562 amino acid proteins. The bioinformatic analysis suggests that the majority of P450 proteins encoded by these genes are membrane anchored in the endoplasmic reticulum. RT-qPCR assays showed differential expression of all CYP genes between male and female. The gene expression was dependent of monoterpenes and exposure time, with some of them sex, antennae and gut region specific. Significant differences among monoterpenes, gut region, antennae and exposure time were found. Our results suggest that some of these genes may be involved in the detoxification process of these compounds during tree colonization.
AB - Bark beetles (Curculionidae: Scolytinae) are major cause of woody plants death in the world. They colonize the stem and other parts of trees recognizing host-produced specific compounds (kairomones) and insect pheromones. Bark beetle's antennae and alimentary canal participate in the host selection identifying chemical compounds produced by trees and insects, and also in the metabolism and detoxification of these compounds. The red turpentine beetle (RTB), Dendroctonus valens LeConte, is an unaggressive species that colonize > 40 pine species (Pinaceae) in North and Central America. Several studies suggest that bark beetle cytochrome P450 enzymes are involved in monoterpene oxidation. In this study we identified by means of PCR, cloning, sequencing, and bioinformatic analysis, eleven full-length genes: five CYP4, four CYP6, and two CYP9 in the antennae and gut region of RTB, after stimulation with vapors of monoterpenes: (±)-α-pinene, (R)-(+)-α-pinene, (S)-(-)-β-pinene, (S)-(-)-α-pinene and (+)-3-carene; pine trees volatiles used by RTB as kairomones. The recovered cDNA of these genes vary from 1.5 kb to 1.8 kb and the open frame encodes from 496 to 562 amino acid proteins. The bioinformatic analysis suggests that the majority of P450 proteins encoded by these genes are membrane anchored in the endoplasmic reticulum. RT-qPCR assays showed differential expression of all CYP genes between male and female. The gene expression was dependent of monoterpenes and exposure time, with some of them sex, antennae and gut region specific. Significant differences among monoterpenes, gut region, antennae and exposure time were found. Our results suggest that some of these genes may be involved in the detoxification process of these compounds during tree colonization.
KW - Communication system
KW - Cytochrome P450
KW - Dendroctonus valens
KW - Detoxification
KW - Expression
UR - http://www.scopus.com/inward/record.url?scp=84875317158&partnerID=8YFLogxK
U2 - 10.1016/j.ibmb.2013.02.004
DO - 10.1016/j.ibmb.2013.02.004
M3 - Artículo
C2 - 23454142
SN - 0965-1748
VL - 43
SP - 417
EP - 432
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 5
ER -