Distribution of virulence genes in clinical and environmental isolates of Aeromonas spp

M. R. Chacón; M. J. Figueras; G. Castro-Escarpulli; L. Soler; J. Guarro

doi:10.1023/A:1026042125243

Distribution of virulence genes in clinical and environmental isolates of Aeromonas spp

M. R. Chacón, M. J. Figueras, G. Castro-Escarpulli, L. Soler, J. Guarro

Escuela Nacional de Ciencias Biológicas (ENCB)

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110 Scopus citations

Abstract

The distribution and phenotypic activity of the genes encoding for serine protease, glycerophospholipid-cholesterol acyltransferase, lipases, aerolysin/hemolysin and DNases were investigated in 234 isolates identified by 16S rDNA-RFLP representing all the species of Aeromonas. The former three genes were found to be highly conserved among the genus. Aerolysin/hemolysin and DNase genes and β-hemolytic activity were significantly more frequent in clinical than in environmental isolates. Aerolysin/hemolysin and serine protease genes were present in all β-hemolytic strains supporting serine protease as possibly important for the activation of the former gene. The high prevalence of virulence factors in clinical isolates indicates that they may play a role in the mechanisms of pathogenesis of these microorganisms.

Original language	English
Pages (from-to)	269-278
Number of pages	10
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	84
Issue number	4
DOIs	https://doi.org/10.1023/A:1026042125243
State	Published - 2003

Keywords

Aerolysin
Aeromonas
DNase
GCAT
Lipases
PCR
Serine protease
Virulence factors

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10.1023/A:1026042125243

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title = "Distribution of virulence genes in clinical and environmental isolates of Aeromonas spp",

abstract = "The distribution and phenotypic activity of the genes encoding for serine protease, glycerophospholipid-cholesterol acyltransferase, lipases, aerolysin/hemolysin and DNases were investigated in 234 isolates identified by 16S rDNA-RFLP representing all the species of Aeromonas. The former three genes were found to be highly conserved among the genus. Aerolysin/hemolysin and DNase genes and β-hemolytic activity were significantly more frequent in clinical than in environmental isolates. Aerolysin/hemolysin and serine protease genes were present in all β-hemolytic strains supporting serine protease as possibly important for the activation of the former gene. The high prevalence of virulence factors in clinical isolates indicates that they may play a role in the mechanisms of pathogenesis of these microorganisms.",

keywords = "Aerolysin, Aeromonas, DNase, GCAT, Lipases, PCR, Serine protease, Virulence factors",

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T1 - Distribution of virulence genes in clinical and environmental isolates of Aeromonas spp

AU - Chacón, M. R.

AU - Figueras, M. J.

AU - Castro-Escarpulli, G.

AU - Soler, L.

AU - Guarro, J.

PY - 2003

Y1 - 2003

N2 - The distribution and phenotypic activity of the genes encoding for serine protease, glycerophospholipid-cholesterol acyltransferase, lipases, aerolysin/hemolysin and DNases were investigated in 234 isolates identified by 16S rDNA-RFLP representing all the species of Aeromonas. The former three genes were found to be highly conserved among the genus. Aerolysin/hemolysin and DNase genes and β-hemolytic activity were significantly more frequent in clinical than in environmental isolates. Aerolysin/hemolysin and serine protease genes were present in all β-hemolytic strains supporting serine protease as possibly important for the activation of the former gene. The high prevalence of virulence factors in clinical isolates indicates that they may play a role in the mechanisms of pathogenesis of these microorganisms.

AB - The distribution and phenotypic activity of the genes encoding for serine protease, glycerophospholipid-cholesterol acyltransferase, lipases, aerolysin/hemolysin and DNases were investigated in 234 isolates identified by 16S rDNA-RFLP representing all the species of Aeromonas. The former three genes were found to be highly conserved among the genus. Aerolysin/hemolysin and DNase genes and β-hemolytic activity were significantly more frequent in clinical than in environmental isolates. Aerolysin/hemolysin and serine protease genes were present in all β-hemolytic strains supporting serine protease as possibly important for the activation of the former gene. The high prevalence of virulence factors in clinical isolates indicates that they may play a role in the mechanisms of pathogenesis of these microorganisms.

KW - Aerolysin

KW - Aeromonas

KW - DNase

KW - GCAT

KW - Lipases

KW - PCR

KW - Serine protease

KW - Virulence factors

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U2 - 10.1023/A:1026042125243

DO - 10.1023/A:1026042125243

M3 - Artículo

SN - 0003-6072

VL - 84

SP - 269

EP - 278

JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

IS - 4

ER -

Distribution of virulence genes in clinical and environmental isolates of Aeromonas spp

Abstract

Keywords

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