TY - JOUR
T1 - Disinfection efficacy of ozone on ESKAPE bacteria biofilms
T2 - Potential use in difficult-to-access medical devices
AU - Ibáñez-Cervantes, Gabriela
AU - Cruz-Cruz, Clemente
AU - Durán-Manuel, Emilio Mariano
AU - Loyola-Cruz, Miguel Ángel
AU - Cureño-Díaz, Mónica Alethia
AU - Castro-Escarpulli, Graciela
AU - Lugo-Zamudio, Gustavo Esteban
AU - Rojo-Gutiérrez, María Isabel
AU - Razo-Blanco Hernández, Dulce Milagros
AU - López-Ornelas, Adolfo
AU - Bello-López, Juan Manuel
N1 - Publisher Copyright:
© 2022 Association for Professionals in Infection Control and Epidemiology, Inc.
PY - 2023/1
Y1 - 2023/1
N2 - Background: Medical devices can be reservoirs of multidrug-resistant bacteria that may be involved in the acquisition of infections since bacteria with the ability to form biofilms that are difficult to eradicate, mainly in mechanical ventilators. The aim of this work was to evaluate the efficacy of O3 against biofilms of bacteria ESKAPE group through disinfection studies. Methods: The formation of biofilms of ESKAPE group bacteria was induced in vitro. O3 was injected at different exposure times at a constant dose of 600 mg/h. The recovery of surviving bacteria after O3 treatment was assessed by bacterial counts and biofilm disruption was analyzed. Finally, the viability and integrity of biofilms after O3 treatment was determined by confocal laser scanning microscopy (CLSM). Results: O3 showed bactericidal activity on biofilms from 12 min/7.68 ppm for A. baumannii and C. freundii. P. aeruginosa, K. pneumoniae and S. aureus were killed after 15 min/9.60 ppm. Correlation analyses showed inversely proportional relationships between the variables "disruption versus O3". CLSM revealed that death was time-dependent of biofilms upon O3 exposure. Orthogonal plane analysis showed that bacteria located in the outer region of the biofilms were the ones that initially suffered damage from O3 exposure. Conclusions: Our findings suggest that this method could be an alternative for the disinfection in mechanical ventilators colonized by bacteria biofilm forming.
AB - Background: Medical devices can be reservoirs of multidrug-resistant bacteria that may be involved in the acquisition of infections since bacteria with the ability to form biofilms that are difficult to eradicate, mainly in mechanical ventilators. The aim of this work was to evaluate the efficacy of O3 against biofilms of bacteria ESKAPE group through disinfection studies. Methods: The formation of biofilms of ESKAPE group bacteria was induced in vitro. O3 was injected at different exposure times at a constant dose of 600 mg/h. The recovery of surviving bacteria after O3 treatment was assessed by bacterial counts and biofilm disruption was analyzed. Finally, the viability and integrity of biofilms after O3 treatment was determined by confocal laser scanning microscopy (CLSM). Results: O3 showed bactericidal activity on biofilms from 12 min/7.68 ppm for A. baumannii and C. freundii. P. aeruginosa, K. pneumoniae and S. aureus were killed after 15 min/9.60 ppm. Correlation analyses showed inversely proportional relationships between the variables "disruption versus O3". CLSM revealed that death was time-dependent of biofilms upon O3 exposure. Orthogonal plane analysis showed that bacteria located in the outer region of the biofilms were the ones that initially suffered damage from O3 exposure. Conclusions: Our findings suggest that this method could be an alternative for the disinfection in mechanical ventilators colonized by bacteria biofilm forming.
KW - Bacterial adherence
KW - Disinfection
KW - Mechanical ventilators
KW - Oxidizing gas
UR - http://www.scopus.com/inward/record.url?scp=85130873033&partnerID=8YFLogxK
U2 - 10.1016/j.ajic.2022.03.037
DO - 10.1016/j.ajic.2022.03.037
M3 - Artículo
C2 - 35483519
AN - SCOPUS:85130873033
SN - 0196-6553
VL - 51
SP - 11
EP - 17
JO - American Journal of Infection Control
JF - American Journal of Infection Control
IS - 1
ER -
