TY - JOUR
T1 - Dipeptidyl aminopeptidase yspI mutants of Schizosaccharomyces pombe
T2 - Genetic mapping of dpa1+ on chromosome III
AU - Villa, Lourdes
AU - Suárez-Rendueles, Paz
N1 - Funding Information:
L. Villa was a fellow of the Fundaci6n para el Fomento en Asturias de la Investigaci6n Cien-tffica Aplicada y la Tecnologia (FICYT). This work was supported in part by grant BIO90/0221 from the Comisi6n Interministerial de Ciencia y Tecnologla. The authors are especially grateful to Dr. A. Dur~in (Instituto de Microbiologla Bioquimica, Universidad de Salamanca, Spain) for providing yeast strains.
PY - 1994/7/1
Y1 - 1994/7/1
N2 - A mutant strain of Schizosaccharomyces pombe lacking dipeptidyl aminopeptidase yspI was isolated from a strain already defective in aminopeptidase activity by means of a staining technique with the chromogenic substrate ala-pro-4-methoxy-β-naphthylamide to screen colonies for the absence of the enzyme. The defect segregated 2+ :2- in meiotic tetrads, indicating a single chromosomal gene mutation, which was shown to be recessive. Gene dosage experiments indicated that the mutation resides in the structural gene of dipeptidyl aminopeptidase yspI, dpa1+. The dpa1+ gene was located on chromosome III by using l m- fluorophen-ylalanine-induced haploidization and mitotic analysis. dpa1 mutants did not show any obvious phenotype under a variety of conditions tested.
AB - A mutant strain of Schizosaccharomyces pombe lacking dipeptidyl aminopeptidase yspI was isolated from a strain already defective in aminopeptidase activity by means of a staining technique with the chromogenic substrate ala-pro-4-methoxy-β-naphthylamide to screen colonies for the absence of the enzyme. The defect segregated 2+ :2- in meiotic tetrads, indicating a single chromosomal gene mutation, which was shown to be recessive. Gene dosage experiments indicated that the mutation resides in the structural gene of dipeptidyl aminopeptidase yspI, dpa1+. The dpa1+ gene was located on chromosome III by using l m- fluorophen-ylalanine-induced haploidization and mitotic analysis. dpa1 mutants did not show any obvious phenotype under a variety of conditions tested.
KW - Fission yeast
KW - Peptidase
KW - Peptidase mutant
KW - Schizosaccharomyces pombe
UR - http://www.scopus.com/inward/record.url?scp=0028365383&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1994.tb07033.x
DO - 10.1111/j.1574-6968.1994.tb07033.x
M3 - Artículo
SN - 0378-1097
VL - 120
SP - 211
EP - 216
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1-2
ER -