TY - JOUR
T1 - Diffusion of Mn2+ ions into liposomes mediated by phosphatidate and monitored by the activation of an encapsulated enzymatic system
AU - Baeza, Isabel
AU - Ibáñez, Miguel
AU - Santiago, J. Carlos
AU - Argüello, Carlos
AU - Wong, Carlos
AU - Oró, J.
PY - 1990/12
Y1 - 1990/12
N2 - Transbilayer diffusion of Mn2+ ions occurred in liposomes formed from dipalmitoyl-phosphatidylcholine or egg-yolk phosphatidylcholine and egg-yolk phosphatidate (molar ratio 2:1) containing DNA and DNase I within their aqueous compartments. Cation diffusion was demonstrated by the hydrolytic activity of DNase I, activated by the Mn2+ ions that diffused into the vesicles, and this was confirmed by light scattering. Phosphatidate, a cone-shaped lipid which has been synthesized under simulated prebiotic conditions, was necessary for cation diffusion across the liposome membranes. Such liposomes represent a simple precellular system that interchanges cations with the surroundings and provides a microenvironment for enzymatic reactions, as evidenced by the hydrolysis of DNA by DNase I inside these closed lipid compartments.
AB - Transbilayer diffusion of Mn2+ ions occurred in liposomes formed from dipalmitoyl-phosphatidylcholine or egg-yolk phosphatidylcholine and egg-yolk phosphatidate (molar ratio 2:1) containing DNA and DNase I within their aqueous compartments. Cation diffusion was demonstrated by the hydrolytic activity of DNase I, activated by the Mn2+ ions that diffused into the vesicles, and this was confirmed by light scattering. Phosphatidate, a cone-shaped lipid which has been synthesized under simulated prebiotic conditions, was necessary for cation diffusion across the liposome membranes. Such liposomes represent a simple precellular system that interchanges cations with the surroundings and provides a microenvironment for enzymatic reactions, as evidenced by the hydrolysis of DNA by DNase I inside these closed lipid compartments.
KW - Cationic transbilayer diffusion
KW - Cone-shaped lipid
KW - Encapsulated enzyme activity
KW - Liposome permeability
KW - Liposomes as a precellular model
KW - Phosphatidate permeability properties
UR - http://www.scopus.com/inward/record.url?scp=0025572642&partnerID=8YFLogxK
U2 - 10.1007/BF02102071
DO - 10.1007/BF02102071
M3 - Artículo
SN - 0022-2844
VL - 31
SP - 453
EP - 461
JO - Journal of Molecular Evolution
JF - Journal of Molecular Evolution
IS - 6
ER -