TY - JOUR
T1 - Differential pattern of cytokine expression by macrophages infected in vitro with different Mycobacterium tuberculosis genotypes
AU - Chacón-Salinas, R.
AU - Serafín-López, J.
AU - Ramos-Payán, R.
AU - Méndez-Aragón, P.
AU - Hernández-Pando, R.
AU - Van Soolingen, D.
AU - Flores-Romo, L.
AU - Estrada-Parra, S.
AU - Estrada-García, Iris
PY - 2005/6
Y1 - 2005/6
N2 - It has been shown recently that different genotypes of Mycobacterium tuberculosis induce distinct immune responses in the host, as reflected by variations in cytokine and iNOS expression. Because these molecules are probably regulated by multiple factors in vivo this complex phenomenon was partially analysed by assessing cytokine and iNOS expression by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) in an in vitro model of bone marrow-derived macrophages infected with three different M. tuberculosis genotypes: Canetti, H37 Rv and Beijing. Although the three genotypes induced production of iNOS and the different cytokines tested at 24 h post-infection, macrophages infected with the Beijing isolate expressed the highest levels of mRNA for iNOS, interleukin (IL)-1β, tumour necrosis factor (TNF)-α, IL-12 cytokines and lower levels of IL-10 compared with cells infected with other genotypes. This expression pattern has been associated with infection control, but during infection in vivo with the Beijing genotype it is lost upon progression to chronic phase. The failure to control infection is likely to be influenced by cytokines produced by other cell types and bacterial molecules expressed during the course of disease. Results presented in this work show that each genotype has the ability to induce different levels of cytokine expression that could be related to its pathogenesis during infection.
AB - It has been shown recently that different genotypes of Mycobacterium tuberculosis induce distinct immune responses in the host, as reflected by variations in cytokine and iNOS expression. Because these molecules are probably regulated by multiple factors in vivo this complex phenomenon was partially analysed by assessing cytokine and iNOS expression by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) in an in vitro model of bone marrow-derived macrophages infected with three different M. tuberculosis genotypes: Canetti, H37 Rv and Beijing. Although the three genotypes induced production of iNOS and the different cytokines tested at 24 h post-infection, macrophages infected with the Beijing isolate expressed the highest levels of mRNA for iNOS, interleukin (IL)-1β, tumour necrosis factor (TNF)-α, IL-12 cytokines and lower levels of IL-10 compared with cells infected with other genotypes. This expression pattern has been associated with infection control, but during infection in vivo with the Beijing genotype it is lost upon progression to chronic phase. The failure to control infection is likely to be influenced by cytokines produced by other cell types and bacterial molecules expressed during the course of disease. Results presented in this work show that each genotype has the ability to induce different levels of cytokine expression that could be related to its pathogenesis during infection.
KW - Bone marrow derived macrophages
KW - Cytokine expression
KW - Mycobacterium tuberculosis genotypes
UR - http://www.scopus.com/inward/record.url?scp=20444498713&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2249.2005.02797.x
DO - 10.1111/j.1365-2249.2005.02797.x
M3 - Artículo
C2 - 15932505
SN - 0009-9104
VL - 140
SP - 443
EP - 449
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 3
ER -