TY - JOUR
T1 - Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display
AU - Rojas-Herrera, R.
AU - Quiroz-Figueroa, F.
AU - Monforte-González, M.
AU - Sánchez-Teyer, L.
AU - Loyola-Vargas, V. M.
PY - 2002
Y1 - 2002
N2 - Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated.
AB - Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated.
KW - Coffea arabica
KW - Differential display
KW - Gene expression
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=0036231017&partnerID=8YFLogxK
U2 - 10.1385/MB:21:1:043
DO - 10.1385/MB:21:1:043
M3 - Artículo
C2 - 11989658
AN - SCOPUS:0036231017
SN - 1073-6085
VL - 21
SP - 43
EP - 50
JO - Molecular Biotechnology
JF - Molecular Biotechnology
IS - 1
ER -