Differential expression of α1-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture

Enrique Mendez; Claudia Calzada; Esther Ocharan; Alfredo Sierra; Carlos Castillo; Israel Ramirez; Eduardo Meaney; Alejandra Meaney; Juan Asbun; Angel Miliar; Jorge Herrera; Guillermo Ceballos

doi:10.1016/j.ejphar.2006.06.070

Differential expression of α₁-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture

Enrique Mendez, Claudia Calzada, Esther Ocharan, Alfredo Sierra, Carlos Castillo, Israel Ramirez, Eduardo Meaney, Alejandra Meaney, Juan Asbun, Angel Miliar, Jorge Herrera, Guillermo Ceballos

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

It has been postulated that in blood vessels under α₁-related stimulation, the endothelial intracellular calcium concentration ([Ca²⁺]_i) increases, which is necessary to induce nitric oxide synthesis, is the result of an increase in vascular smooth muscle, which subsequently, flows into the endothelial cells through gap junctions and it is not the result of a direct adrenergic stimulation of endothelial receptors. Others, however, postulate that endothelial α_1D-adrenoceptors, have a direct effect on nitric oxide synthesis. In order to clarify this phenomena, in this work we analyzed the presence of α₁receptor subtypes and their functional association with nitric oxide synthesis in rat coronary microvascular endothelial cells in culture, with pharmacological, immunological and reverse transcriptase polymerase chain reaction approaches. Our results show the presence and functional coupling with nitric oxide synthesis of α_1A and α_1D-adrenoceptor subtypes. α_1B-adrenoceptor subtype is not coupled with nitric oxide production.

Original language	English
Pages (from-to)	127-133
Number of pages	7
Journal	European Journal of Pharmacology
Volume	546
Issue number	1-3
DOIs	https://doi.org/10.1016/j.ejphar.2006.06.070
State	Published - 28 Sep 2006
Externally published	Yes

Keywords

Endothelium
Nitric Oxide
α1-Adrenoceptor subtype

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10.1016/j.ejphar.2006.06.070

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Mendez, E., Calzada, C., Ocharan, E., Sierra, A., Castillo, C., Ramirez, I., Meaney, E., Meaney, A., Asbun, J., Miliar, A., Herrera, J., & Ceballos, G. (2006). Differential expression of α₁-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture. European Journal of Pharmacology, 546(1-3), 127-133. https://doi.org/10.1016/j.ejphar.2006.06.070

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title = "Differential expression of α1-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture",

abstract = "It has been postulated that in blood vessels under α1-related stimulation, the endothelial intracellular calcium concentration ([Ca2+]i) increases, which is necessary to induce nitric oxide synthesis, is the result of an increase in vascular smooth muscle, which subsequently, flows into the endothelial cells through gap junctions and it is not the result of a direct adrenergic stimulation of endothelial receptors. Others, however, postulate that endothelial α1D-adrenoceptors, have a direct effect on nitric oxide synthesis. In order to clarify this phenomena, in this work we analyzed the presence of α1receptor subtypes and their functional association with nitric oxide synthesis in rat coronary microvascular endothelial cells in culture, with pharmacological, immunological and reverse transcriptase polymerase chain reaction approaches. Our results show the presence and functional coupling with nitric oxide synthesis of α1A and α1D-adrenoceptor subtypes. α1B-adrenoceptor subtype is not coupled with nitric oxide production.",

keywords = "Endothelium, Nitric Oxide, α1-Adrenoceptor subtype",

author = "Enrique Mendez and Claudia Calzada and Esther Ocharan and Alfredo Sierra and Carlos Castillo and Israel Ramirez and Eduardo Meaney and Alejandra Meaney and Juan Asbun and Angel Miliar and Jorge Herrera and Guillermo Ceballos",

note = "Funding Information: This work was supported by a CONACyT grant #46187/A and I.P.N.",

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Mendez, E, Calzada, C , Ocharan, E , Sierra, A, Castillo, C, Ramirez, I , Meaney, E, Meaney, A, Asbun, J, Miliar, A, Herrera, J & Ceballos, G 2006, 'Differential expression of α₁-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture', European Journal of Pharmacology, vol. 546, no. 1-3, pp. 127-133. https://doi.org/10.1016/j.ejphar.2006.06.070

TY - JOUR

T1 - Differential expression of α1-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture

AU - Mendez, Enrique

AU - Calzada, Claudia

AU - Ocharan, Esther

AU - Sierra, Alfredo

AU - Castillo, Carlos

AU - Ramirez, Israel

AU - Meaney, Eduardo

AU - Meaney, Alejandra

AU - Asbun, Juan

AU - Miliar, Angel

AU - Herrera, Jorge

AU - Ceballos, Guillermo

N1 - Funding Information: This work was supported by a CONACyT grant #46187/A and I.P.N.

PY - 2006/9/28

Y1 - 2006/9/28

N2 - It has been postulated that in blood vessels under α1-related stimulation, the endothelial intracellular calcium concentration ([Ca2+]i) increases, which is necessary to induce nitric oxide synthesis, is the result of an increase in vascular smooth muscle, which subsequently, flows into the endothelial cells through gap junctions and it is not the result of a direct adrenergic stimulation of endothelial receptors. Others, however, postulate that endothelial α1D-adrenoceptors, have a direct effect on nitric oxide synthesis. In order to clarify this phenomena, in this work we analyzed the presence of α1receptor subtypes and their functional association with nitric oxide synthesis in rat coronary microvascular endothelial cells in culture, with pharmacological, immunological and reverse transcriptase polymerase chain reaction approaches. Our results show the presence and functional coupling with nitric oxide synthesis of α1A and α1D-adrenoceptor subtypes. α1B-adrenoceptor subtype is not coupled with nitric oxide production.

AB - It has been postulated that in blood vessels under α1-related stimulation, the endothelial intracellular calcium concentration ([Ca2+]i) increases, which is necessary to induce nitric oxide synthesis, is the result of an increase in vascular smooth muscle, which subsequently, flows into the endothelial cells through gap junctions and it is not the result of a direct adrenergic stimulation of endothelial receptors. Others, however, postulate that endothelial α1D-adrenoceptors, have a direct effect on nitric oxide synthesis. In order to clarify this phenomena, in this work we analyzed the presence of α1receptor subtypes and their functional association with nitric oxide synthesis in rat coronary microvascular endothelial cells in culture, with pharmacological, immunological and reverse transcriptase polymerase chain reaction approaches. Our results show the presence and functional coupling with nitric oxide synthesis of α1A and α1D-adrenoceptor subtypes. α1B-adrenoceptor subtype is not coupled with nitric oxide production.

KW - Endothelium

KW - Nitric Oxide

KW - α1-Adrenoceptor subtype

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U2 - 10.1016/j.ejphar.2006.06.070

DO - 10.1016/j.ejphar.2006.06.070

M3 - Artículo

SN - 0014-2999

VL - 546

SP - 127

EP - 133

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-3

ER -

Differential expression of α₁-adrenergic receptor subtypes in coronary microvascular endothelial cells in culture

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Keywords

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