TY - JOUR
T1 - Differential CD4+ T-cell memory responses induced by two subsets of human monocyte-derived dendritic cells
AU - Bajaña, Sandra
AU - Herrera-González, Norma
AU - Narváez, Juana
AU - Torres-Aguilar, Honorio
AU - Rivas-Carvalho, Amaranta
AU - Aguilar, Sergio R.
AU - Sánchez-Torres, Carmen
PY - 2007/11
Y1 - 2007/11
N2 - Dendritic cells (DC) are powerful inducers of primary T-cell responses, but their role in secondary responses has not been extensively analysed. Here, we address the role of two DC subsets derived from human CD16+ (16 + mDC) or CD16- (16- mDC) monocytes on the reactivation of memory responses. CD4+ CD45RA- memory T cells were obtained from adult blood donors, and central (TCM) and effector (TEM) memory T cells were isolated by fluorescence-activated cell sorting with anti-CCR7 antibodies. The 16+ mDC and 16 - mDC were cocultured with autologous lymphocytes, either unpulsed or loaded with purified protein derivatives of Mycobacterium tuberculosis (PPD) or tetanus toxoid (TT), and were analysed for up to 8 days. Over a range of doses, 16+ mDC drove stronger T-cell proliferative responses against both antigens. Overall, antigen-specific memory cells tended to acquire a phenotype of TEM at later time-points in the culture, whereas cells that had completed fewer cycles of division were similar to TCM. The 16 + mDC induced higher rates of proliferation on both TCM and TEM lymphocytes than 16- mDC. This phenomenon was not related to the ability of both DC to induce CD25 expression on T cells, to lower secretion of interleukin-2, or to raise production of interleukin-10 during T-cell/16- mDC cocultures. The induction of TCM effector capacity in terms of interferon-γ production was faster and more pronounced with 16+ mDC, whereas both DC had similar abilities with TEM. In conclusion, these data might reveal new potentials in vaccination protocols with 16+ mDC aimed at inducing strong responses on central memory T cells.
AB - Dendritic cells (DC) are powerful inducers of primary T-cell responses, but their role in secondary responses has not been extensively analysed. Here, we address the role of two DC subsets derived from human CD16+ (16 + mDC) or CD16- (16- mDC) monocytes on the reactivation of memory responses. CD4+ CD45RA- memory T cells were obtained from adult blood donors, and central (TCM) and effector (TEM) memory T cells were isolated by fluorescence-activated cell sorting with anti-CCR7 antibodies. The 16+ mDC and 16 - mDC were cocultured with autologous lymphocytes, either unpulsed or loaded with purified protein derivatives of Mycobacterium tuberculosis (PPD) or tetanus toxoid (TT), and were analysed for up to 8 days. Over a range of doses, 16+ mDC drove stronger T-cell proliferative responses against both antigens. Overall, antigen-specific memory cells tended to acquire a phenotype of TEM at later time-points in the culture, whereas cells that had completed fewer cycles of division were similar to TCM. The 16 + mDC induced higher rates of proliferation on both TCM and TEM lymphocytes than 16- mDC. This phenomenon was not related to the ability of both DC to induce CD25 expression on T cells, to lower secretion of interleukin-2, or to raise production of interleukin-10 during T-cell/16- mDC cocultures. The induction of TCM effector capacity in terms of interferon-γ production was faster and more pronounced with 16+ mDC, whereas both DC had similar abilities with TEM. In conclusion, these data might reveal new potentials in vaccination protocols with 16+ mDC aimed at inducing strong responses on central memory T cells.
KW - Dendritic cell subsets
KW - Interferon-γ production
KW - Lymphoproliferation
KW - Recall antigens
UR - http://www.scopus.com/inward/record.url?scp=35048896449&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2567.2007.02650.x
DO - 10.1111/j.1365-2567.2007.02650.x
M3 - Artículo
SN - 0019-2805
VL - 122
SP - 381
EP - 393
JO - Immunology
JF - Immunology
IS - 3
ER -