TY - JOUR
T1 - Differential aromatase (CYP19) expression in human arteries from normal and neoplasic uterus
T2 - An immunohistochemical and in situ hybridization study
AU - Calzada-Mendoza, Claudia C.
AU - Sánchez, C. Elena
AU - Campos, R. Rafael
AU - Becerril, M. Adriana
AU - Madrigal, B. Eduardo
AU - Sierra, R. Alfredo
AU - Mendez, B. Enrique
AU - Ocharán, H. E.
AU - Herrera, G. Norma
AU - Guillermo, Ceballos Reyes
PY - 2006
Y1 - 2006
N2 - Aromatase CYP19 catalyzes the synthesis of estrogen from androgens in a tissue-specific manner. This enzyme is present in several tissues, including gonads, brain and fatty tissue. More recently, its presence has been described in vessels. Here, we describe the expression of aromatase in human uterine artery and compare its expression with that found in arteries of estrogen-dependent uterine leiomyomata from women. To do this, we employed immunohystochemical and in situ hybridization techniques. We used, a polyclonal antibody raised against the carboxyl terminus of aromatase (ARO) and RNAm probes, of the exon 1 of ARO. We found an increased immunoreactivity of ARO in uterine arteries of patients with leiomyoma as compared with control group. Probe showing positive signal in skin fibroblasts (1b), showed positive hybridization signal in normal artery, while probes with positive signal in placenta (1a), ovary (1c) and test's (1d) were over-expressed in arteries of leiomyomas.
AB - Aromatase CYP19 catalyzes the synthesis of estrogen from androgens in a tissue-specific manner. This enzyme is present in several tissues, including gonads, brain and fatty tissue. More recently, its presence has been described in vessels. Here, we describe the expression of aromatase in human uterine artery and compare its expression with that found in arteries of estrogen-dependent uterine leiomyomata from women. To do this, we employed immunohystochemical and in situ hybridization techniques. We used, a polyclonal antibody raised against the carboxyl terminus of aromatase (ARO) and RNAm probes, of the exon 1 of ARO. We found an increased immunoreactivity of ARO in uterine arteries of patients with leiomyoma as compared with control group. Probe showing positive signal in skin fibroblasts (1b), showed positive hybridization signal in normal artery, while probes with positive signal in placenta (1a), ovary (1c) and test's (1d) were over-expressed in arteries of leiomyomas.
KW - Aromatase
KW - CYP19 c
KW - Expression
KW - Immunohistochemical staining
KW - In situ Hybridization
KW - Leiomyoma
KW - Userus
UR - http://www.scopus.com/inward/record.url?scp=32844474894&partnerID=8YFLogxK
U2 - 10.2741/1806
DO - 10.2741/1806
M3 - Artículo
SN - 2768-6701
VL - 11
SP - 389
EP - 393
JO - Frontiers in Bioscience
JF - Frontiers in Bioscience
IS - 1 P.1-446
ER -