TY - JOUR
T1 - Developmental Effects of Amoxicillin at Environmentally Relevant Concentration Using Zebrafish Embryotoxicity Test (ZET)
AU - González-González, Edgar David
AU - Gómez-Oliván, Leobardo Manuel
AU - Islas-Flores, Hariz
AU - Galar-Martínez, Marcela
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2021/5
Y1 - 2021/5
N2 - Abstract: Amoxicillin (AMX) is an antibiotic that has been added to the watch list of substances by the European Union, through the Water Framework Directive (WFD; 2000/60/EC) and its daughter regulation (Decision 2018/840) for which monitoring data have to be gathered with a possibility for future regulation. Previous studies have demonstrated that this antibiotic generates toxic effects, among which oxidative stress in aquatic organisms is noteworthy. The aim of this study was to evaluate the effect on embryonic development and the teratogenic effects induced by AMX at environmentally relevant concentrations in oocytes and embryos of Danio rerio. Furthermore, oxidative stress biomarkers were evaluated at 72 and 96 hpf. The LC50 was 14.192 μgL−1, EC50 was 7.083 μgL−1, and TI was 2.003. Biomarkers of cellular oxidation and antioxidant enzymes were modified in a concentration-dependent way with respect to the control group (p<0.05). The main malformations identified were tail malformation, pericardial edema, yolk sac malformation, scoliosis, pectoral fin absence, and no hatching. The results allow us to conclude that AMX at environmentally relevant concentrations is capable of inducing embryotoxic and teratogenic effects and oxidative damage. This compound represents a risk to aquatic organisms such as Danio rerio. Clinical Trials Registration: Not applicable.
AB - Abstract: Amoxicillin (AMX) is an antibiotic that has been added to the watch list of substances by the European Union, through the Water Framework Directive (WFD; 2000/60/EC) and its daughter regulation (Decision 2018/840) for which monitoring data have to be gathered with a possibility for future regulation. Previous studies have demonstrated that this antibiotic generates toxic effects, among which oxidative stress in aquatic organisms is noteworthy. The aim of this study was to evaluate the effect on embryonic development and the teratogenic effects induced by AMX at environmentally relevant concentrations in oocytes and embryos of Danio rerio. Furthermore, oxidative stress biomarkers were evaluated at 72 and 96 hpf. The LC50 was 14.192 μgL−1, EC50 was 7.083 μgL−1, and TI was 2.003. Biomarkers of cellular oxidation and antioxidant enzymes were modified in a concentration-dependent way with respect to the control group (p<0.05). The main malformations identified were tail malformation, pericardial edema, yolk sac malformation, scoliosis, pectoral fin absence, and no hatching. The results allow us to conclude that AMX at environmentally relevant concentrations is capable of inducing embryotoxic and teratogenic effects and oxidative damage. This compound represents a risk to aquatic organisms such as Danio rerio. Clinical Trials Registration: Not applicable.
KW - Amoxicillin
KW - Embryotoxicity
KW - Teratogenicity
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85105196892&partnerID=8YFLogxK
U2 - 10.1007/s11270-021-05148-6
DO - 10.1007/s11270-021-05148-6
M3 - Artículo
AN - SCOPUS:85105196892
SN - 0049-6979
VL - 232
JO - Water, Air, and Soil Pollution
JF - Water, Air, and Soil Pollution
IS - 5
M1 - 196
ER -