TY - JOUR
T1 - Development of microsatellite loci for the Honduran white-bat (Ectophylla alba) by using Illumina paired-end sequences
AU - Morales-Leyva, Alberto
AU - Medellín, Rodrigo A.
AU - Lance, Stacey L.
AU - Rodríguez-Herrera, Bernal
AU - Del Real-Monroy, Melina
AU - Ortega, Jorge
N1 - Publisher Copyright:
© 2013, Springer Science+Business Media Dordrecht.
PY - 2014/3/1
Y1 - 2014/3/1
N2 - Ectophylla alba is a bat restricted to Costa Rica, Honduras, Nicaragua and Panamá. A technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats was used to develop loci. Thirteen polymorphic (tri, tetra- and pentanucleotide) microsatellites were developed and tested. All markers were genotyped on 42 different individuals from 5 distinct locations. We observed low to medium–high genetic variation across most loci. Levels of expected heterozygosity across all markers was medium to low (mean HE = 0.659, mean HO = 0.672).
AB - Ectophylla alba is a bat restricted to Costa Rica, Honduras, Nicaragua and Panamá. A technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats was used to develop loci. Thirteen polymorphic (tri, tetra- and pentanucleotide) microsatellites were developed and tested. All markers were genotyped on 42 different individuals from 5 distinct locations. We observed low to medium–high genetic variation across most loci. Levels of expected heterozygosity across all markers was medium to low (mean HE = 0.659, mean HO = 0.672).
KW - Ectophylla alba
KW - Illumina
KW - Microsatellites
KW - Pal_finder
UR - http://www.scopus.com/inward/record.url?scp=84926340490&partnerID=8YFLogxK
U2 - 10.1007/s12686-013-0065-8
DO - 10.1007/s12686-013-0065-8
M3 - Carta
SN - 1877-7252
VL - 6
SP - 219
EP - 220
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 1
ER -