TY - JOUR
T1 - Development of digestive enzymes in larvae of Mayan cichlid Cichlasoma urophthalmus
AU - López-Ramírez, G.
AU - Cuenca-Soria, C. A.
AU - Alvarez-González, C. A.
AU - Tovar-Ramírez, D.
AU - Ortiz-Galindo, J. L.
AU - Perales-García, N.
AU - Márquez-Couturier, G.
AU - Arias-Rodríguez, L.
AU - Indy, J. R.
AU - Contreras-Sánchez, W. M.
AU - Gisbert, E.
AU - Moyano, F. J.
N1 - Funding Information:
This study was financed by FOMIX CONACYT-Government of the State of Tabasco, through the research project TAB-2005-C06-16260 and partial funding for this research was provided by the Aquaculture Collaborative Research Support. Program accession number # 1373. The Aquaculture CRSP is funded in part by United States Agency for International Development (USAID) Grant No. LAG-G-00-96-90015-00 and by participating institutions. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of the US Agency of International Development.
Funding Information:
Acknowledgments First author thanks to the Programa Estatal de Nuevos Talentos, of the Consejo de Ciencia y Tecnología, of the state of Tabasco for the fellowship grant.
PY - 2011/3
Y1 - 2011/3
N2 - The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparticulated trout food (45% protein and 16% lipids) from 16 to 60 dah. Several samples were collected including yolk-sac larvae (considered as day 1 after hatching) and specimens up to 60 dah. Most digestive enzymes were present from yolk absorption (5-6 dah), except for the specific acid proteases activity (pepsin-like), which increase rapidly from 8 dah up to 20 dah. Three alkaline proteases isoforms (24.0, 24.8, 84.5 kDa) were detected at 8 dah using SDS-PAGE zymogram, corresponding to trypsin, chymotrypsin and probably leucine aminopeptidase enzymes, and only one isoform was detected (relative electromobility, Rf = 0.54) for acid proteases (pepsin-like) from 3 dah onwards using PAGE zymogram. We concluded that C. urophthamus is a precocious fish with a great capacity to digest all kinds of food items, including artificial diets provided from 13 dah.
AB - The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparticulated trout food (45% protein and 16% lipids) from 16 to 60 dah. Several samples were collected including yolk-sac larvae (considered as day 1 after hatching) and specimens up to 60 dah. Most digestive enzymes were present from yolk absorption (5-6 dah), except for the specific acid proteases activity (pepsin-like), which increase rapidly from 8 dah up to 20 dah. Three alkaline proteases isoforms (24.0, 24.8, 84.5 kDa) were detected at 8 dah using SDS-PAGE zymogram, corresponding to trypsin, chymotrypsin and probably leucine aminopeptidase enzymes, and only one isoform was detected (relative electromobility, Rf = 0.54) for acid proteases (pepsin-like) from 3 dah onwards using PAGE zymogram. We concluded that C. urophthamus is a precocious fish with a great capacity to digest all kinds of food items, including artificial diets provided from 13 dah.
KW - Cichlasoma urophthalmus
KW - Lipase
KW - Mayan cichlid
KW - Phosphatase
KW - Zymogram
KW - α-Amylase
UR - http://www.scopus.com/inward/record.url?scp=79952574559&partnerID=8YFLogxK
U2 - 10.1007/s10695-010-9431-6
DO - 10.1007/s10695-010-9431-6
M3 - Artículo
SN - 0920-1742
VL - 37
SP - 197
EP - 208
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
IS - 1
ER -