Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis

C. A. Alvarez-González; F. J. Moyano-López; R. Civera-Cerecedo; V. Carrasco-Chávez; J. L. Ortíz-Galindo; H. Nolasco-Soria; D. Tovar-Ramírez; S. Dumas

doi:10.1007/s10695-008-9276-4

Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis

C. A. Alvarez-González, F. J. Moyano-López, R. Civera-Cerecedo, V. Carrasco-Chávez, J. L. Ortíz-Galindo, H. Nolasco-Soria, D. Tovar-Ramírez, S. Dumas

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27 Scopus citations

Abstract

The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like band from day 12 ah onwards. In addition, two lipase bands first appeared on day 2 ah, and there was a change in the molecular weight of one band from day 15 ah onwards. Several α-amylase isoforms were observed from hatching up to day 5 ah. These results indicate that the important digestive enzymes develop rapidly in these larvae, supporting the possibility of early weaning at day 12 ah using artificial diets.

Original language	English
Pages (from-to)	29-37
Number of pages	9
Journal	Fish Physiology and Biochemistry
Volume	36
Issue number	1
DOIs	https://doi.org/10.1007/s10695-008-9276-4
State	Published - Mar 2010
Externally published	Yes

Keywords

Electrophoresis
Lipase
Ontogeny
Proteases
Spotted sand bass
Zymogram
α-amylase

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10.1007/s10695-008-9276-4

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Alvarez-González, C. A., Moyano-López, F. J., Civera-Cerecedo, R., Carrasco-Chávez, V., Ortíz-Galindo, J. L., Nolasco-Soria, H., Tovar-Ramírez, D., & Dumas, S. (2010). Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis. Fish Physiology and Biochemistry, 36(1), 29-37. https://doi.org/10.1007/s10695-008-9276-4

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title = "Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis",

abstract = "The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like band from day 12 ah onwards. In addition, two lipase bands first appeared on day 2 ah, and there was a change in the molecular weight of one band from day 15 ah onwards. Several α-amylase isoforms were observed from hatching up to day 5 ah. These results indicate that the important digestive enzymes develop rapidly in these larvae, supporting the possibility of early weaning at day 12 ah using artificial diets.",

keywords = "Electrophoresis, Lipase, Ontogeny, Proteases, Spotted sand bass, Zymogram, α-amylase",

author = "Alvarez-Gonz{\'a}lez, {C. A.} and Moyano-L{\'o}pez, {F. J.} and R. Civera-Cerecedo and V. Carrasco-Ch{\'a}vez and Ort{\'i}z-Galindo, {J. L.} and H. Nolasco-Soria and D. Tovar-Ram{\'i}rez and S. Dumas",

note = "Funding Information: Acknowledgements This work was supported by Instituto Polit{\'e}cnico Nacional projects: CGPI 998022, 20010825, and 20020357, the Consejo Nacional de Ciencia y Tecnolog{\'i}a of Mexico (31666B), the Universidad de Almer{\'i}a, and the Centro de Investigaciones Biol{\'o}gicas del Noroeste.",

year = "2010",

month = mar,

doi = "10.1007/s10695-008-9276-4",

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volume = "36",

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journal = "Fish Physiology and Biochemistry",

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number = "1",

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Alvarez-González, CA, Moyano-López, FJ, Civera-Cerecedo, R, Carrasco-Chávez, V, Ortíz-Galindo, JL, Nolasco-Soria, H, Tovar-Ramírez, D & Dumas, S 2010, 'Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis', Fish Physiology and Biochemistry, vol. 36, no. 1, pp. 29-37. https://doi.org/10.1007/s10695-008-9276-4

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T1 - Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II

T2 - Electrophoretic analysis

AU - Alvarez-González, C. A.

AU - Moyano-López, F. J.

AU - Civera-Cerecedo, R.

AU - Carrasco-Chávez, V.

AU - Ortíz-Galindo, J. L.

AU - Nolasco-Soria, H.

AU - Tovar-Ramírez, D.

AU - Dumas, S.

N1 - Funding Information: Acknowledgements This work was supported by Instituto Politécnico Nacional projects: CGPI 998022, 20010825, and 20020357, the Consejo Nacional de Ciencia y Tecnología of Mexico (31666B), the Universidad de Almería, and the Centro de Investigaciones Biológicas del Noroeste.

PY - 2010/3

Y1 - 2010/3

N2 - The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like band from day 12 ah onwards. In addition, two lipase bands first appeared on day 2 ah, and there was a change in the molecular weight of one band from day 15 ah onwards. Several α-amylase isoforms were observed from hatching up to day 5 ah. These results indicate that the important digestive enzymes develop rapidly in these larvae, supporting the possibility of early weaning at day 12 ah using artificial diets.

AB - The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like band from day 12 ah onwards. In addition, two lipase bands first appeared on day 2 ah, and there was a change in the molecular weight of one band from day 15 ah onwards. Several α-amylase isoforms were observed from hatching up to day 5 ah. These results indicate that the important digestive enzymes develop rapidly in these larvae, supporting the possibility of early weaning at day 12 ah using artificial diets.

KW - Electrophoresis

KW - Lipase

KW - Ontogeny

KW - Proteases

KW - Spotted sand bass

KW - Zymogram

KW - α-amylase

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DO - 10.1007/s10695-008-9276-4

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SN - 0920-1742

VL - 36

SP - 29

EP - 37

JO - Fish Physiology and Biochemistry

JF - Fish Physiology and Biochemistry

IS - 1

ER -

Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis

Abstract

Keywords

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