Development of a UPLC-MS/MS method for the quantification of sildenafil by DBS, and its use on pediatric pulmonary hypertension

Leticia Contreras Zavala; Liliana Rivera Espinosa; Alionka P.Ángeles Moreno; Eduardo Ramírez San-Juan; Sergio Zamudio Hernández; Alberto García González; Gabriel Marcelín Jiménez

doi:10.4155/bio.14.126

Development of a UPLC-MS/MS method for the quantification of sildenafil by DBS, and its use on pediatric pulmonary hypertension

Leticia Contreras Zavala, Liliana Rivera Espinosa, Alionka P.Ángeles Moreno, Eduardo Ramírez San-Juan, Sergio Zamudio Hernández, Alberto García González, Gabriel Marcelín Jiménez

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Background: Sildenafil is used for the treatment of pediatric pulmonary hypertension. A dried blood spot (DBS)-based LC-MS method for sildenafil quantitation was developed and applied to a group of patients. Results: DBS showed high portability and stability of samples, and the method was selective and linear for quantitation of sildenafil (5-3,000 ng/ml) and N-desmethyl-sildenafil (3-1,500 ng/ml). After a single oral dose of sildenafil (1 mg/kg), method evidenced poor metabolism in these patients.Conclusion: The method was successfully applied in peripheral blood and can be used for both pharmacokinetics and therapeutic drug monitoring. DBS proved to have advantages during sample translation and preservation of analytes. Data suggest that hazardous blood sildenafil levels may be reached in this population.

Original language	English
Pages (from-to)	2815-2824
Number of pages	10
Journal	Bioanalysis
Volume	6
Issue number	21
DOIs	https://doi.org/10.4155/bio.14.126
State	Published - 1 Nov 2014

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title = "Development of a UPLC-MS/MS method for the quantification of sildenafil by DBS, and its use on pediatric pulmonary hypertension",

abstract = "Background: Sildenafil is used for the treatment of pediatric pulmonary hypertension. A dried blood spot (DBS)-based LC-MS method for sildenafil quantitation was developed and applied to a group of patients. Results: DBS showed high portability and stability of samples, and the method was selective and linear for quantitation of sildenafil (5-3,000 ng/ml) and N-desmethyl-sildenafil (3-1,500 ng/ml). After a single oral dose of sildenafil (1 mg/kg), method evidenced poor metabolism in these patients.Conclusion: The method was successfully applied in peripheral blood and can be used for both pharmacokinetics and therapeutic drug monitoring. DBS proved to have advantages during sample translation and preservation of analytes. Data suggest that hazardous blood sildenafil levels may be reached in this population.",

author = "Zavala, {Leticia Contreras} and Espinosa, {Liliana Rivera} and Moreno, {Alionka P.{\'A}ngeles} and San-Juan, {Eduardo Ram{\'i}rez} and Hern{\'a}ndez, {Sergio Zamudio} and Gonz{\'a}lez, {Alberto Garc{\'i}a} and Jim{\'e}nez, {Gabriel Marcel{\'i}n}",

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year = "2014",

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doi = "10.4155/bio.14.126",

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T1 - Development of a UPLC-MS/MS method for the quantification of sildenafil by DBS, and its use on pediatric pulmonary hypertension

AU - Zavala, Leticia Contreras

AU - Espinosa, Liliana Rivera

AU - Moreno, Alionka P.Ángeles

AU - San-Juan, Eduardo Ramírez

AU - Hernández, Sergio Zamudio

AU - González, Alberto García

AU - Jiménez, Gabriel Marcelín

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Background: Sildenafil is used for the treatment of pediatric pulmonary hypertension. A dried blood spot (DBS)-based LC-MS method for sildenafil quantitation was developed and applied to a group of patients. Results: DBS showed high portability and stability of samples, and the method was selective and linear for quantitation of sildenafil (5-3,000 ng/ml) and N-desmethyl-sildenafil (3-1,500 ng/ml). After a single oral dose of sildenafil (1 mg/kg), method evidenced poor metabolism in these patients.Conclusion: The method was successfully applied in peripheral blood and can be used for both pharmacokinetics and therapeutic drug monitoring. DBS proved to have advantages during sample translation and preservation of analytes. Data suggest that hazardous blood sildenafil levels may be reached in this population.

AB - Background: Sildenafil is used for the treatment of pediatric pulmonary hypertension. A dried blood spot (DBS)-based LC-MS method for sildenafil quantitation was developed and applied to a group of patients. Results: DBS showed high portability and stability of samples, and the method was selective and linear for quantitation of sildenafil (5-3,000 ng/ml) and N-desmethyl-sildenafil (3-1,500 ng/ml). After a single oral dose of sildenafil (1 mg/kg), method evidenced poor metabolism in these patients.Conclusion: The method was successfully applied in peripheral blood and can be used for both pharmacokinetics and therapeutic drug monitoring. DBS proved to have advantages during sample translation and preservation of analytes. Data suggest that hazardous blood sildenafil levels may be reached in this population.

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DO - 10.4155/bio.14.126

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SN - 1757-6180

VL - 6

SP - 2815

EP - 2824

JO - Bioanalysis

JF - Bioanalysis

IS - 21

ER -

Development of a UPLC-MS/MS method for the quantification of sildenafil by DBS, and its use on pediatric pulmonary hypertension

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