Determinación de tween-20 en AgsHB por un método espectrofotométrico

Emilio Medina Rivero; Carlos A. López M.; Néstor O. Pérez R.; Igor Ferlan

Determinación de tween-20 en AgsHB por un método espectrofotométrico

Translated title of the contribution: Determination of tween-20 in AgsHB through a spectrophotometric method

Emilio Medina Rivero, Carlos A. López M., Néstor O. Pérez R., Igor Ferlan

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

tween-20 is used during cell disruption to obtain Hepatitis B virus surface antigen (HBsAg). The specifications for HBsAg indicate that the quantity of Tween-20 allowed must not be more than 50 mg for each 100 mg of purified protein. Therefore, Tween-20 quantification must be determined before the release of each batch. This study proposes a spectrophotometric method to estimate the Tween-20 concentration in purified AgsHB samples, followed to the formation of a cobalt - polyoxyethylene complex, and its measure at 620 nm of optical density. This work focuses on the validation of this method.

Translated title of the contribution	Determination of tween-20 in AgsHB through a spectrophotometric method
Original language	Spanish
Pages (from-to)	11-15
Number of pages	5
Journal	Revista Mexicana de Ciencias Farmaceuticas
Volume	39
Issue number	2
State	Published - Apr 2008
Externally published	Yes

Cite this

@article{164e76c3f95c4b45ac2ae79c7ff7a7f4,

title = "Determinaci{\'o}n de tween-20 en AgsHB por un m{\'e}todo espectrofotom{\'e}trico",

abstract = "tween-20 is used during cell disruption to obtain Hepatitis B virus surface antigen (HBsAg). The specifications for HBsAg indicate that the quantity of Tween-20 allowed must not be more than 50 mg for each 100 mg of purified protein. Therefore, Tween-20 quantification must be determined before the release of each batch. This study proposes a spectrophotometric method to estimate the Tween-20 concentration in purified AgsHB samples, followed to the formation of a cobalt - polyoxyethylene complex, and its measure at 620 nm of optical density. This work focuses on the validation of this method.",

keywords = "HBsAg, Spectrophotometry, Tween-20, Validation",

author = "{Medina Rivero}, Emilio and {L{\'o}pez M.}, {Carlos A.} and {P{\'e}rez R.}, {N{\'e}stor O.} and Igor Ferlan",

year = "2008",

month = apr,

language = "Espa{\~n}ol",

volume = "39",

pages = "11--15",

journal = "Revista Mexicana de Ciencias Farmaceuticas",

issn = "1027-3956",

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}

TY - JOUR

T1 - Determinación de tween-20 en AgsHB por un método espectrofotométrico

AU - Medina Rivero, Emilio

AU - López M., Carlos A.

AU - Pérez R., Néstor O.

AU - Ferlan, Igor

PY - 2008/4

Y1 - 2008/4

N2 - tween-20 is used during cell disruption to obtain Hepatitis B virus surface antigen (HBsAg). The specifications for HBsAg indicate that the quantity of Tween-20 allowed must not be more than 50 mg for each 100 mg of purified protein. Therefore, Tween-20 quantification must be determined before the release of each batch. This study proposes a spectrophotometric method to estimate the Tween-20 concentration in purified AgsHB samples, followed to the formation of a cobalt - polyoxyethylene complex, and its measure at 620 nm of optical density. This work focuses on the validation of this method.

AB - tween-20 is used during cell disruption to obtain Hepatitis B virus surface antigen (HBsAg). The specifications for HBsAg indicate that the quantity of Tween-20 allowed must not be more than 50 mg for each 100 mg of purified protein. Therefore, Tween-20 quantification must be determined before the release of each batch. This study proposes a spectrophotometric method to estimate the Tween-20 concentration in purified AgsHB samples, followed to the formation of a cobalt - polyoxyethylene complex, and its measure at 620 nm of optical density. This work focuses on the validation of this method.

KW - HBsAg

KW - Spectrophotometry

KW - Tween-20

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=46749104563&partnerID=8YFLogxK

M3 - Artículo

SN - 1027-3956

VL - 39

SP - 11

EP - 15

JO - Revista Mexicana de Ciencias Farmaceuticas

JF - Revista Mexicana de Ciencias Farmaceuticas

IS - 2

ER -

Determinación de tween-20 en AgsHB por un método espectrofotométrico

Abstract

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