TY - JOUR
T1 - Detection of the effects of Methylobacterium in Agave tequilana Weber var. azul by laser-induced fluorescence
AU - Cervantes-Martínez, Jesús
AU - López-Díaz, Sigifredo
AU - Rodríguez-Garay, Benjamín
N1 - Funding Information:
S. López-Dı́az is a graduate student at Posgrado en Procesos Biotecnológicos, U. de G., CIATEJ, A.C. and financially supported by CONACYT-Mexico.
PY - 2004/4
Y1 - 2004/4
N2 - The laser-induced fluorescence (LIF) was tested as a means to detect the effects of a pink-pigmented facultative methylotrophic bacteria (Methylobacterium) in blue agave plants, and compared with conventional techniques of leaf tissue analysis. A He-Ne laser emitting at 632.8nm was used as the excitation source. In vivo fluorescence emission spectra were recorded in the 660-790nm range. Fluorescence maxima were at 690 and 740nm. The inoculated plants showed a significant change in fluorescence as compared to that of non-inoculated plants. The ratios of fluorescence intensities F 690/F740 were calculated for all treatments. A comparison between conventional techniques and this non-invasive method is presented. The results of this work showed that the beneficial effects of PPFM bacteria on agave plants are detectable by LIF.
AB - The laser-induced fluorescence (LIF) was tested as a means to detect the effects of a pink-pigmented facultative methylotrophic bacteria (Methylobacterium) in blue agave plants, and compared with conventional techniques of leaf tissue analysis. A He-Ne laser emitting at 632.8nm was used as the excitation source. In vivo fluorescence emission spectra were recorded in the 660-790nm range. Fluorescence maxima were at 690 and 740nm. The inoculated plants showed a significant change in fluorescence as compared to that of non-inoculated plants. The ratios of fluorescence intensities F 690/F740 were calculated for all treatments. A comparison between conventional techniques and this non-invasive method is presented. The results of this work showed that the beneficial effects of PPFM bacteria on agave plants are detectable by LIF.
KW - Emission
KW - Fluorescence
KW - Fluorescence intensity
KW - PPFM
KW - Spectra
UR - http://www.scopus.com/inward/record.url?scp=17144472438&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2003.11.029
DO - 10.1016/j.plantsci.2003.11.029
M3 - Artículo
AN - SCOPUS:17144472438
SN - 0168-9452
VL - 166
SP - 889
EP - 892
JO - Plant Science
JF - Plant Science
IS - 4
ER -