TY - JOUR
T1 - Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma
T2 - A comparative study in children and adults
AU - Palma, Icela
AU - Sánchez, Ana Elena
AU - Jiménez-Hernández, Elva
AU - Alvarez-Rodríguez, Francisco
AU - Nava-Frias, Margarita
AU - Valencia-Mayoral, Pedro
AU - Salinas-Lara, Citlatepet
AU - Velazquez-Guadarrama, Norma
AU - Portilla-Aguilar, Josefina
AU - Pena, Rocio Y.
AU - Ramos-Salazar, Patricia
AU - Contreras, Alejandra
AU - Alfaro, Ana
AU - Espinosa, Ana Maria
AU - Nájera, Nayelli
AU - Gutierrez, Gisela
AU - Mejia-Arangure, Juan Manuel
AU - Arellano-Galindo, José
N1 - Funding Information:
This work was supported by Proyecto ESM-IPN SIP: 20091798, 20101275, 20113149, CONACYT: 2007/C01/71285, CB-2007-1-83949, HIMFG register: HIM/2008/031
PY - 2013/6
Y1 - 2013/6
N2 - Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals - 42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P =.41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P =.22). A mix of subtypes was found in 4/21 (19%) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.
AB - Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals - 42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P =.41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P =.22). A mix of subtypes was found in 4/21 (19%) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.
KW - DNA
KW - EBER
KW - EBNA2A
KW - EBNA2B
KW - Epstein-Barr virus
KW - In situ hybridization
KW - LMP
UR - http://www.scopus.com/inward/record.url?scp=84877776426&partnerID=8YFLogxK
U2 - 10.1016/j.clml.2012.11.010
DO - 10.1016/j.clml.2012.11.010
M3 - Artículo
C2 - 23276887
SN - 2152-2650
VL - 13
SP - 266
EP - 272
JO - Clinical Lymphoma, Myeloma and Leukemia
JF - Clinical Lymphoma, Myeloma and Leukemia
IS - 3
ER -