Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma: A comparative study in children and adults

Icela Palma, Ana Elena Sánchez, Elva Jiménez-Hernández, Francisco Alvarez-Rodríguez, Margarita Nava-Frias, Pedro Valencia-Mayoral, Citlatepet Salinas-Lara, Norma Velazquez-Guadarrama, Josefina Portilla-Aguilar, Rocio Y. Pena, Patricia Ramos-Salazar, Alejandra Contreras, Ana Alfaro, Ana Maria Espinosa, Nayelli Nájera, Gisela Gutierrez, Juan Manuel Mejia-Arangure, José Arellano-Galindo

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Abstract

Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals - 42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P =.41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P =.22). A mix of subtypes was found in 4/21 (19%) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.
Original languageAmerican English
Pages (from-to)266-272
Number of pages238
JournalClinical Lymphoma, Myeloma and Leukemia
DOIs
StatePublished - 1 Jun 2013

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Human Herpesvirus 4
Hodgkin Disease
Proteins
Epstein-Barr Virus Nuclear Antigens
Herpesviridae
Paraffin
In Situ Hybridization
Lymph Nodes
Genotype
RNA
Biopsy
Polymerase Chain Reaction

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Palma, Icela ; Sánchez, Ana Elena ; Jiménez-Hernández, Elva ; Alvarez-Rodríguez, Francisco ; Nava-Frias, Margarita ; Valencia-Mayoral, Pedro ; Salinas-Lara, Citlatepet ; Velazquez-Guadarrama, Norma ; Portilla-Aguilar, Josefina ; Pena, Rocio Y. ; Ramos-Salazar, Patricia ; Contreras, Alejandra ; Alfaro, Ana ; Espinosa, Ana Maria ; Nájera, Nayelli ; Gutierrez, Gisela ; Mejia-Arangure, Juan Manuel ; Arellano-Galindo, José. / Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma: A comparative study in children and adults. In: Clinical Lymphoma, Myeloma and Leukemia. 2013 ; pp. 266-272.
@article{7ef66ef02ce540789806757b4960af95,
title = "Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma: A comparative study in children and adults",
abstract = "Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals - 42 (63{\%}) from children and 24 (37{\%}) from adults with HL. Thirty-two of the 42 samples (76.1{\%}) were positive for EBV in children and 16 of 24 (66.6{\%}) samples were positive in adults (P =.41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8{\%}). EBNA2A was found in 7/21 (33.3{\%}) children and in 4/13 (30.8{\%}) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6{\%}) children and in 9/13 (69.2{\%}) adults (P =.22). A mix of subtypes was found in 4/21 (19{\%}) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.",
author = "Icela Palma and S{\'a}nchez, {Ana Elena} and Elva Jim{\'e}nez-Hern{\'a}ndez and Francisco Alvarez-Rodr{\'i}guez and Margarita Nava-Frias and Pedro Valencia-Mayoral and Citlatepet Salinas-Lara and Norma Velazquez-Guadarrama and Josefina Portilla-Aguilar and Pena, {Rocio Y.} and Patricia Ramos-Salazar and Alejandra Contreras and Ana Alfaro and Espinosa, {Ana Maria} and Nayelli N{\'a}jera and Gisela Gutierrez and Mejia-Arangure, {Juan Manuel} and Jos{\'e} Arellano-Galindo",
year = "2013",
month = "6",
day = "1",
doi = "10.1016/j.clml.2012.11.010",
language = "American English",
pages = "266--272",
journal = "Clinical Lymphoma, Myeloma and Leukemia",
issn = "2152-2650",
publisher = "Cancer Media Group",

}

Palma, I, Sánchez, AE, Jiménez-Hernández, E, Alvarez-Rodríguez, F, Nava-Frias, M, Valencia-Mayoral, P, Salinas-Lara, C, Velazquez-Guadarrama, N, Portilla-Aguilar, J, Pena, RY, Ramos-Salazar, P, Contreras, A, Alfaro, A, Espinosa, AM, Nájera, N, Gutierrez, G, Mejia-Arangure, JM & Arellano-Galindo, J 2013, 'Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma: A comparative study in children and adults', Clinical Lymphoma, Myeloma and Leukemia, pp. 266-272. https://doi.org/10.1016/j.clml.2012.11.010

Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma: A comparative study in children and adults. / Palma, Icela; Sánchez, Ana Elena; Jiménez-Hernández, Elva; Alvarez-Rodríguez, Francisco; Nava-Frias, Margarita; Valencia-Mayoral, Pedro; Salinas-Lara, Citlatepet; Velazquez-Guadarrama, Norma; Portilla-Aguilar, Josefina; Pena, Rocio Y.; Ramos-Salazar, Patricia; Contreras, Alejandra; Alfaro, Ana; Espinosa, Ana Maria; Nájera, Nayelli; Gutierrez, Gisela; Mejia-Arangure, Juan Manuel; Arellano-Galindo, José.

In: Clinical Lymphoma, Myeloma and Leukemia, 01.06.2013, p. 266-272.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in mexican patients with Hodgkin lymphoma: A comparative study in children and adults

AU - Palma, Icela

AU - Sánchez, Ana Elena

AU - Jiménez-Hernández, Elva

AU - Alvarez-Rodríguez, Francisco

AU - Nava-Frias, Margarita

AU - Valencia-Mayoral, Pedro

AU - Salinas-Lara, Citlatepet

AU - Velazquez-Guadarrama, Norma

AU - Portilla-Aguilar, Josefina

AU - Pena, Rocio Y.

AU - Ramos-Salazar, Patricia

AU - Contreras, Alejandra

AU - Alfaro, Ana

AU - Espinosa, Ana Maria

AU - Nájera, Nayelli

AU - Gutierrez, Gisela

AU - Mejia-Arangure, Juan Manuel

AU - Arellano-Galindo, José

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals - 42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P =.41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P =.22). A mix of subtypes was found in 4/21 (19%) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.

AB - Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals - 42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P =.41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P =.22). A mix of subtypes was found in 4/21 (19%) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.

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U2 - 10.1016/j.clml.2012.11.010

DO - 10.1016/j.clml.2012.11.010

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JO - Clinical Lymphoma, Myeloma and Leukemia

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