TY - JOUR
T1 - Detección de malatión en larvas de dípteros de importancia forense en el noreste de méxico
AU - Estrada-Camacho, Israel
AU - Hernández-Mendoza, José Luis
AU - Torres-Ortega, Jorge
AU - Quiroz-Velázquez, Jesús Di Carlo
AU - Villarreal-Sotelo, Karla
N1 - Publisher Copyright:
© 2020 Sociedad Colombiana de Entomología-SOCOLEN y Universidad del Valle-Univalle.
PY - 2020
Y1 - 2020
N2 - Acute pesticide poisoning is a global public health problem. In developing countries pesticides cause up to one million cases of poisoning and up to 20,000 deaths annually. In Mexico, it has been reported that due to intoxications by xenobiotics, among them pesticides, 1,400 people die annually. In the present work a method was optimized to detect and quantify malathion in third instar fly larvae (Diptera) that hatched in pork meat, Sus scrofa domesticus, treated with the lethal dose in humans of malathion (60 g/60 kg). A biomass of 1.5 g which was added 1.2 mL of phase, stirred by space 5 min and centrifuged for 10 min at 10.000 rpm. The supernatant was analyzed by HPLC, using a methanol-water phase (70/30). The retention time of malathion was 4.1 min. The detection limit was calculated at 0.301 ppm. The technique and procedure used will serve as an indirect tool to identify the presence of the toxin in a relatively simple way in court cases.
AB - Acute pesticide poisoning is a global public health problem. In developing countries pesticides cause up to one million cases of poisoning and up to 20,000 deaths annually. In Mexico, it has been reported that due to intoxications by xenobiotics, among them pesticides, 1,400 people die annually. In the present work a method was optimized to detect and quantify malathion in third instar fly larvae (Diptera) that hatched in pork meat, Sus scrofa domesticus, treated with the lethal dose in humans of malathion (60 g/60 kg). A biomass of 1.5 g which was added 1.2 mL of phase, stirred by space 5 min and centrifuged for 10 min at 10.000 rpm. The supernatant was analyzed by HPLC, using a methanol-water phase (70/30). The retention time of malathion was 4.1 min. The detection limit was calculated at 0.301 ppm. The technique and procedure used will serve as an indirect tool to identify the presence of the toxin in a relatively simple way in court cases.
KW - Diptera
KW - Entomotoxicology
KW - Insecticide
KW - Intoxication
KW - Organophosphorus
UR - http://www.scopus.com/inward/record.url?scp=85089400174&partnerID=8YFLogxK
U2 - 10.25100/SOCOLEN.V46I1.10166
DO - 10.25100/SOCOLEN.V46I1.10166
M3 - Artículo
AN - SCOPUS:85089400174
SN - 0120-0488
VL - 46
SP - 1
EP - 5
JO - Revista Colombiana de Entomologia
JF - Revista Colombiana de Entomologia
IS - 1
M1 - e10166
ER -