TY - JOUR
T1 - Dengue virus serotype-2 impairs proliferation of healthy donors' T Lymphocytes
AU - Fuentes-Miranda, C. J.
AU - Sánchez-García, F. J.
AU - Coker, A. R.
AU - Rojas-Espinosa, O.
AU - Salinas-Tobón, R.
AU - Moreno-Altamirano, M. M.B.
PY - 2014/3
Y1 - 2014/3
N2 - Objectives: T lymphocytes are not infected by dengue virus (DENV), nevertheless it is possible that exposure to DENV may affect their function. T lymphocytes from DENV-infected individuals are impaired in their proliferative capacity, although this effect has been attributed to altered function of antigen-presenting cells rather than to an intrinsic defect on T lymphocytes. Here we analyzed whether T lymphocytes from healthy donors became impaired in their proliferative capacity following in vitro exposure to DENV serotype-2 (DENV-2), as well as the possible mechanisms for this. Methods: Isolated CD4+ and CD8+ T lymphocytes from healthy donors were in vitro exposed to DENV-2, before polyclonal activation, cell proliferation, IL-2 synthesis. IL-2R expression, nuclear translocation of NF-AT and NF-κB, and intracellular calcium flux were assessed. Results: In vitro exposure of both CD4+ and CD8+ T lymphocytes from healthy donors to DENV-2 impairs cell proliferation, IL-2 synthesis, and IL-2R (CD25) cell membrane expression. Signalling wise, exposure to DENV-2 impairs the nuclear translocation of NF-AT, downstream of intracellular calcium mobilization, as well as that of NF-κB. Conclusion: In the course of a dengue infection, direct exposure of T lymphocytes to DENV could affect cell-mediated immune responses.
AB - Objectives: T lymphocytes are not infected by dengue virus (DENV), nevertheless it is possible that exposure to DENV may affect their function. T lymphocytes from DENV-infected individuals are impaired in their proliferative capacity, although this effect has been attributed to altered function of antigen-presenting cells rather than to an intrinsic defect on T lymphocytes. Here we analyzed whether T lymphocytes from healthy donors became impaired in their proliferative capacity following in vitro exposure to DENV serotype-2 (DENV-2), as well as the possible mechanisms for this. Methods: Isolated CD4+ and CD8+ T lymphocytes from healthy donors were in vitro exposed to DENV-2, before polyclonal activation, cell proliferation, IL-2 synthesis. IL-2R expression, nuclear translocation of NF-AT and NF-κB, and intracellular calcium flux were assessed. Results: In vitro exposure of both CD4+ and CD8+ T lymphocytes from healthy donors to DENV-2 impairs cell proliferation, IL-2 synthesis, and IL-2R (CD25) cell membrane expression. Signalling wise, exposure to DENV-2 impairs the nuclear translocation of NF-AT, downstream of intracellular calcium mobilization, as well as that of NF-κB. Conclusion: In the course of a dengue infection, direct exposure of T lymphocytes to DENV could affect cell-mediated immune responses.
KW - Cell proliferation
KW - Dengue virus
KW - NF-AT
KW - NF-κB
KW - T lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=84898599023&partnerID=8YFLogxK
U2 - 10.1159/000357180
DO - 10.1159/000357180
M3 - Artículo
C2 - 24480857
SN - 0300-5526
VL - 57
SP - 83
EP - 92
JO - Intervirology
JF - Intervirology
IS - 2
ER -