TY - JOUR
T1 - Decolourization of direct blue 2 by peroxidases obtained from an industrial soybean waste
AU - Miranda-Mandujano, E.
AU - Moeller-Chávez, G.
AU - Villegas-Rosas, O.
AU - Buitrón, Germán
AU - Garzón-Zúñiga, M. A.
N1 - Publisher Copyright:
© 2018, South African Water Research Commission. All rights reserved.
PY - 2018/4
Y1 - 2018/4
N2 - In this work the decolourization of Direct Blue 2 dye (DB2) using an industrial soybean waste as a source of peroxidases was studied. Temperature, pH, amount of H2 O2 and concentration of dye were evaluated to determine the maximum catalytic activity of the enzyme during the dye degradation process. It was observed that a temperature of 40°C, a pH of 5 and a concentration of 40 mg/L for the dye in aqueous phase, play a significant role in the overall enzyme-mediated reaction. The maximum decolourization efficiency achieved under optimal conditions was 70% ± 4%. HPLC studies were carried out to confirm dye degradation and analyse the intermediate metabolites. The oxidation products quantified during the reaction were benzidine and 4 aminobiphenyl. Also, an increase in toxicity, determined by Vibrio fisheri, was observed after the enzymatic oxidation of the dye. Results suggest that the oxidation of DB2 with peroxidases can be recommended as a pretreatment step before a conventional treatment process.
AB - In this work the decolourization of Direct Blue 2 dye (DB2) using an industrial soybean waste as a source of peroxidases was studied. Temperature, pH, amount of H2 O2 and concentration of dye were evaluated to determine the maximum catalytic activity of the enzyme during the dye degradation process. It was observed that a temperature of 40°C, a pH of 5 and a concentration of 40 mg/L for the dye in aqueous phase, play a significant role in the overall enzyme-mediated reaction. The maximum decolourization efficiency achieved under optimal conditions was 70% ± 4%. HPLC studies were carried out to confirm dye degradation and analyse the intermediate metabolites. The oxidation products quantified during the reaction were benzidine and 4 aminobiphenyl. Also, an increase in toxicity, determined by Vibrio fisheri, was observed after the enzymatic oxidation of the dye. Results suggest that the oxidation of DB2 with peroxidases can be recommended as a pretreatment step before a conventional treatment process.
KW - Decolourization
KW - Direct Blue 2
KW - Industrial waste
KW - Oxidation
KW - Soybean peroxidase
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=85045972976&partnerID=8YFLogxK
U2 - 10.4314/wsa.v44i2.06
DO - 10.4314/wsa.v44i2.06
M3 - Artículo
SN - 0378-4738
VL - 44
SP - 204
EP - 210
JO - Water SA
JF - Water SA
IS - 2
ER -