Abstract
Textile dyes contamination represent a global environmental problem. In this paper it is shown that discoloration of Direct Brown 2 (DB2) can be obtained using crude extracts of intracellular and extracellular enzymes and fungal biomass from Trametes versicolor cultured in liquid media with metallics inductors in its viable form. The experimental series were run at room temperature, 800 rpm and initial pH 4.5. Laccase activity was determined in each of the extracts. The discoloration percentage was determined by UV-Visible spectrophotometry. Assays were performed by triplicate. The use of extracellular enzymes such as laccase, lignin and manganese peroxidase led to the total discoloration of 50mg·l-1 of DB2, after 88h; while the use of active mycelial biomass, allowed reaching a total discoloration after 3h for an initial DB2concentration of 100mg·-1. It was found that DB2 removal efficiency with mycelia is comparable or higher than the one reported with immobilized Phanerochaete chrysosporium.
| Translated title of the contribution | Decoloration of DB2 (Direct Brown 2) by intracelular and extracelular enzyme from Trametes versicolor |
|---|---|
| Original language | Spanish |
| Pages (from-to) | 294-298 |
| Number of pages | 5 |
| Journal | Interciencia |
| Volume | 37 |
| Issue number | 4 |
| State | Published - Apr 2012 |