TY - JOUR
T1 - Cytotoxic effect and apoptotic activity of Parmentiera edulis DC. hexane extract on the breast cancer cell line MDA-MB-231
AU - Gómez, Cynthia Carolina Estanislao
AU - Ordaz, Cynthia Pichardo
AU - San Martín, Eduardo Martínez
AU - Pérez, Nury Hernández
AU - Pérez, Guillermo Ishiwara
AU - Gómez, María del Consuelo García
N1 - Publisher Copyright:
© 2016 Cynthia Carolina Estanislao Gómez et al.
PY - 2016
Y1 - 2016
N2 - Parmentiera edulis is a traditional medicine plant used for different purposes in Mexico; however its biological effects and their potential mechanisms have not been sufficiently investigated. In the present work, the in vitro cytotoxic and pro-apoptotic potential effects of hexane extract of bark of P. edulis on the MDA-MB-231 breast cancer cell line were studied. The hexane extract elicited the most pronounced cytotoxic effect in breast cancer cells, as evaluated by MTT assays performed 72 h after treatment, showing an IC50 value of 94.63 + 1.63 μg/ml. No effects were observed in the immortalized mammary epithelial MCF10A cell line and lymphocytes. The anti-proliferative activity observed following P. edulis treatment was associated with morphological changes, DNA fragmentation, phosphatidyl serine externalization and activation of caspases 3 and 9, all of them typical characteristics of apoptosis. Our findings demonstrate specific cytotoxic activity of P. edulis and the apoptosis induction through the intrinsic pathway in the breast cancer cells. Further research is necessary to find out the effective secondary metabolites responsible of cell death induction.
AB - Parmentiera edulis is a traditional medicine plant used for different purposes in Mexico; however its biological effects and their potential mechanisms have not been sufficiently investigated. In the present work, the in vitro cytotoxic and pro-apoptotic potential effects of hexane extract of bark of P. edulis on the MDA-MB-231 breast cancer cell line were studied. The hexane extract elicited the most pronounced cytotoxic effect in breast cancer cells, as evaluated by MTT assays performed 72 h after treatment, showing an IC50 value of 94.63 + 1.63 μg/ml. No effects were observed in the immortalized mammary epithelial MCF10A cell line and lymphocytes. The anti-proliferative activity observed following P. edulis treatment was associated with morphological changes, DNA fragmentation, phosphatidyl serine externalization and activation of caspases 3 and 9, all of them typical characteristics of apoptosis. Our findings demonstrate specific cytotoxic activity of P. edulis and the apoptosis induction through the intrinsic pathway in the breast cancer cells. Further research is necessary to find out the effective secondary metabolites responsible of cell death induction.
KW - Apoptosis
KW - Cancer
KW - Caspases
KW - MDA-MB-231
KW - Parmentiera edulis
UR - http://www.scopus.com/inward/record.url?scp=84957618576&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2016.600103
DO - 10.7324/JAPS.2016.600103
M3 - Artículo
SN - 2231-3354
VL - 6
SP - 15
EP - 22
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 1
ER -