TY - JOUR
T1 - Cytotoxic activity of the methanolic extract of turnera diffusa willd on breast cancer cells
AU - Avelino-Flores, María Del Carmen
AU - Cruz-López, María Del Carmen
AU - Jiménez-Montejo, Fabiola E.
AU - Reyes-Leyva, Julio
N1 - Publisher Copyright:
© Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2015.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Turnera diffusa Willd, commonly known as Damiana, is employed in traditional medicine as a stimulant, aphrodisiac, and diuretic. Its leaves and stems are used for flavoring and infusion. Damiana is considered to be safe for medicinal use by the FDA. Pharmacological studies have established the hypoglycemic, antiaromatase, prosexual, estrogenic, antibacterial, and antioxidant activity of T. diffusa. The aim of the present study was to evaluate the possible cytotoxic effect of extracts and organic fractions of this plant on five tumor cell lines (SiHa, C-33, Hep G2, MDA-MB-231, and T-47D) and normal human fibroblasts. The results show that the methanolic extract (TdM) displayed greater activity on MDA-MB-231 breast cancer cells (with an IC50 of 30.67μg/mL) than on the other cancer cell lines. Four organic fractions of this extract exhibited activity on this cancer cell line. In the most active fraction (F4), two active compounds were isolated, arbutin (1) and apigenin (2). This is the first report of a cytotoxic effect by T. diffusa on cancer cells. The IC50 values suggest that the methanolic extract of T. diffusa has potential as an anticancer therapy.
AB - Turnera diffusa Willd, commonly known as Damiana, is employed in traditional medicine as a stimulant, aphrodisiac, and diuretic. Its leaves and stems are used for flavoring and infusion. Damiana is considered to be safe for medicinal use by the FDA. Pharmacological studies have established the hypoglycemic, antiaromatase, prosexual, estrogenic, antibacterial, and antioxidant activity of T. diffusa. The aim of the present study was to evaluate the possible cytotoxic effect of extracts and organic fractions of this plant on five tumor cell lines (SiHa, C-33, Hep G2, MDA-MB-231, and T-47D) and normal human fibroblasts. The results show that the methanolic extract (TdM) displayed greater activity on MDA-MB-231 breast cancer cells (with an IC50 of 30.67μg/mL) than on the other cancer cell lines. Four organic fractions of this extract exhibited activity on this cancer cell line. In the most active fraction (F4), two active compounds were isolated, arbutin (1) and apigenin (2). This is the first report of a cytotoxic effect by T. diffusa on cancer cells. The IC50 values suggest that the methanolic extract of T. diffusa has potential as an anticancer therapy.
KW - Damiana
KW - MDA-MB-231 cells
KW - T-47D cells
KW - apigenin
KW - breast cancer
KW - cytotoxic activity
KW - fibroblast
UR - http://www.scopus.com/inward/record.url?scp=84924134152&partnerID=8YFLogxK
U2 - 10.1089/jmf.2013.0055
DO - 10.1089/jmf.2013.0055
M3 - Artículo
C2 - 25299247
SN - 1096-620X
VL - 18
SP - 299
EP - 305
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 3
ER -