TY - JOUR
T1 - Cyclic AMP regulates the biosynthesis of cellobiohydrolase in Cellulomonas Xavigena growing in sugar cane bagasse
AU - Herrera-Herrera, Jesús Antonio
AU - Pérez-Avalos, Odilia
AU - Salgado, Luis M.
AU - Ponce-Noyola, Teresa
N1 - Funding Information:
Acknowledgments This work was supported by grant 45678-Z from Consejo Nacional de Ciencia y Tecnología (Conacyt), Mexico. JAHH was supported by PhD fellowship from Conacyt. The authors thank M. Mercado-Morales for technical assistance.
PY - 2009
Y1 - 2009
N2 - Cellulomonas Xavigena produces a battery of cellulase components that act concertedly to degrade cellulose. The addition of cAMP to repressed C. Xavigena cultures released catabolic repression, while addition of cAMP to induced C. Xavigena cultures led to a cellobiohydrolase hyperproduction. Exogenous cAMP showed positive regulation on cellobiohydrolase production in C. Xavigena grown on sugar cane bagasse. A C. Xavigena cellobiohydrolase gene was cloned (named celA), which coded for a 71- kDa enzyme. Upstream, a repressor celR1, identiWed as a 38 kDa protein, was monitored by use of polyclonal antibodies.
AB - Cellulomonas Xavigena produces a battery of cellulase components that act concertedly to degrade cellulose. The addition of cAMP to repressed C. Xavigena cultures released catabolic repression, while addition of cAMP to induced C. Xavigena cultures led to a cellobiohydrolase hyperproduction. Exogenous cAMP showed positive regulation on cellobiohydrolase production in C. Xavigena grown on sugar cane bagasse. A C. Xavigena cellobiohydrolase gene was cloned (named celA), which coded for a 71- kDa enzyme. Upstream, a repressor celR1, identiWed as a 38 kDa protein, was monitored by use of polyclonal antibodies.
KW - CAMP regulation
KW - Cellobiohydrolase
KW - Cellulomonas Xavigena
UR - http://www.scopus.com/inward/record.url?scp=77950470495&partnerID=8YFLogxK
U2 - 10.1007/s00203-009-0502-y
DO - 10.1007/s00203-009-0502-y
M3 - Artículo
SN - 0302-8933
VL - 191
SP - 745
EP - 750
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 10
ER -