TY - JOUR
T1 - Cupriavidus and Burkholderia species associated with agricultural plants that grow in alkaline soils
AU - Estrada-de los Santos, Paulina
AU - Vacaseydel-Aceves, Nora Belinda
AU - Martínez-Aguilar, Lourdes
AU - Cruz-Hernández, María Antonia
AU - Mendoza-Herrera, Alberto
AU - Caballero-Mellado, Jesús
N1 - Funding Information:
This work was partially supported by Instituto Politecnico Nacional Grant SIP 20080500. We are grateful to Silvia Susana Fernandez Davila for technical support. We thank our colleague and coauthor Dr. Jesús Caballero Mellado, who is sadly no longer with us, for many years of friendship, teaching, support and fruitful collaboration.
PY - 2011/12
Y1 - 2011/12
N2 - The presence of Burkholderia, Cupriavidus, and Ralstonia species in northeastern Mexico was investigated. An analysis of the root surrounding soil from different agricultural plants led to the isolation of Burkholderia and Cupriavidus species but no Ralstonia strains. Most Cupriavidus species were unknown and grouped into two clusters according to ARDRA profiles. The 16S rRNA sequence analysis showed that the Cupriavidus isolates were highly related among them and with different Cupriavidus species with validated names. However, SDS-PAGE profiles were distinct among the different ARDRA profiles and to other Cupriavidus species examined, suggesting new species in the genus. This shows that Cupriavidus is more widely associated with plants than previously appreciated. The BCC isolate was 99% similar to B. cenocepacia by recA sequence analysis. Additionally, most Cupriavidus strains from the two largest groups grew on media containing up to 0.1 mg/ml of copper, 10.0 mg/ml arsenic and 1.0 mg/ml zinc. Burkholderia strains grew on media containing up to 10.0 mg/ml zinc, 5.0 mg/ml arsenic and 0.1 mg/ml copper.
AB - The presence of Burkholderia, Cupriavidus, and Ralstonia species in northeastern Mexico was investigated. An analysis of the root surrounding soil from different agricultural plants led to the isolation of Burkholderia and Cupriavidus species but no Ralstonia strains. Most Cupriavidus species were unknown and grouped into two clusters according to ARDRA profiles. The 16S rRNA sequence analysis showed that the Cupriavidus isolates were highly related among them and with different Cupriavidus species with validated names. However, SDS-PAGE profiles were distinct among the different ARDRA profiles and to other Cupriavidus species examined, suggesting new species in the genus. This shows that Cupriavidus is more widely associated with plants than previously appreciated. The BCC isolate was 99% similar to B. cenocepacia by recA sequence analysis. Additionally, most Cupriavidus strains from the two largest groups grew on media containing up to 0.1 mg/ml of copper, 10.0 mg/ml arsenic and 1.0 mg/ml zinc. Burkholderia strains grew on media containing up to 10.0 mg/ml zinc, 5.0 mg/ml arsenic and 0.1 mg/ml copper.
KW - Burkholderia
KW - Ralsonia
KW - heavy metal
KW - plant-associated bacteria
KW - soil PH
UR - http://www.scopus.com/inward/record.url?scp=84555188540&partnerID=8YFLogxK
U2 - 10.1007/s12275-011-1127-9
DO - 10.1007/s12275-011-1127-9
M3 - Artículo
C2 - 22203548
AN - SCOPUS:84555188540
SN - 1225-8873
VL - 49
SP - 867
EP - 876
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 6
ER -